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25 questions
Which type of restriction endonuclease is used in recombinant DNA technology
The enzymes used to cut and join DNA are
Enzyme that removes a phosphate group from 5' ends of DNA
Acid phosphatase
Lambda exonuclease
Alkaline phosphatase
Terminal transferase
Enzyme that can add homopolymer tails to 3' ends of DNA
Terminal transferase
DNA polymerase
Lambda exonuclease
Polynucleotide kinase
Name a restriction Endonuclease (no space in between)
Type II Restriction Endonucleases recognise....
.,....... Sequence
Enzyme that can introduce a small gap of 2 to 3 nucleotides in DNA are
S1 nuclease
Restriction Endonuclease
Lambda exonuclease
Lambda exonuclease cleaved DNA is an excellent substrate for the enzyme
DNA polymerase
Terminal transferase
Polynucleotide kinase
Alkaline phosphatase
Enzyme that forms a phosphodiester bond between 3'OH of one DNA strand and 5'P of another DNA strand
A Restriction Endonuclease (without spacing) that creates a 3' overhang
Klenov fragment of DNA polymerase has which of following activities
5' to 3' polymerisation
5' to 3' exonuclease
3' to 5' exonuclease
Enzyme that use ATP and Mg as cofactor and can ligate both DNA and RNA
T4 DNA ligase
E coli DNA ligase
Restriction endonuclease with same sequence specificity and cut site
Isoshizomer
Neoschizomer
Isocaudomer
Enzyme used in the modification of bases
SmaI and XmaI are
Isoshizomer
Neoschizomer
Isocaudomer
At high pH if restriction endonuclease cleaves a similar sequence but not identical to their defined recognition sequence is called
Enzyme used to join cohesive ends, blunt ends and linkers
E coli DNA ligase
T4 DNA ligase
Topoisomerase
Enzyme that can phophorylate 5' end of DNA
Terminal transferase
Polynucleotide kinase
Alkaline phosphatase
Lambda exonuclease
An enzyme that is used to identify exons
Lambda exonuclease
S1 nuclease
Terminal transferase
Restriction Endonuclease
Enzyme used in RNA labelling
DNA polymerase
Taq polymerase
RNA polymerase
Topoisomerase
Enzyme with high processivity
E coli DNA polymerase
T4 DNA polymerase
T7 RNA polymerase
Taq polymerase
Enzyme used in PCR
T4 DNA polymerase
T7 DNA polymerase
Taq polymerase
T7 RNA polymerase
Thermophilic polymerase with lowest error rate
Taq
Vent
Pfu
Exo Klenov fragment has
5' to 3' exonuclease activity
3' to 5' exonuclease activity
5' to 3' polymerization activity
All the above
Enzyme that is used to prevent recirculation of plasmid and dimer formation
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