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- 1. Multiple Choice
A technique for making millions of copies of a specific region of DNA.
DNA ligase
restriction enzymes
gel electrophoresis
polymerase chain reaction
- 2. Multiple Choice
From where does Taq polymerase come?
Bacteria that live in hydrothermal vents/hot springs.
Cambodia
The rain forest
The CDC
- 3. Multiple ChoiceWhat happens in the Denature step of PCR?The DNA nucleotides are broken apart.The base-pairing rules for DNA are reversed.The double-stranded DNA is separated into two single strands of DNA.The DNA is returned to its natural setting.
- 4. Multiple Choice
At what temperature does the Denature step of PCR occur?
95° C
50° C
72° C
Any temperature
- 5. Multiple Choice
At what temperature does the Anneal step of PCR occur?
95° C
50° C
72° C
Any temperature
- 6. Multiple Choice
At what temperature does the Extension step of PCR occur?
95°C
50-65°C
72°C
Any temperature
- 7. Multiple ChoiceWhat happens during the Anneal step of PCR?Primers are created.The primers attach to the target DNA region.Primers copy the new DNA strand.Primers sequence DNA
- 8. Multiple ChoiceWhat is the function of a primer?To identify the particular region of DNA to be copied by PCR.To copy DNA.To create DNA nucleotides.To maintain the temperature of the PCR reaction.
- 9. Multiple ChoiceWhat is the purpose of the Extension step of PCR?To create the primers.To extend the time it takes to produce DNA.To allow polymerase to create the complementary strands of DNA.To allow substrates to copy DNA sequences.
- 10. Multiple ChoiceWhy does PCR require a specific type of polymerase?Taq polymerase can withstand the range of temperatures in PCR without denaturing.Taq polymerase is the most inexpensive type of polymerase.Taq polymerase is the most common type of polymerase.A specific type of polymerase is unnecessary.
- 11. Multiple ChoiceHow many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length?1234
- 12. Multiple ChoiceWhat type of bonds hold the two strands of DNA together?Hydrogen bondsCovalent bondsDipole-Dipole bondsIonic bonds
- 13. Multiple ChoiceWhat are the base-pairing rules in DNA?A-C, G-TA-U, G-CA-T, G-CA-C, G-U
- 14. Multiple Choice
If the primer sequence is TCG, what section of DNA will it anneal?
AGC
CAT
GAC
ATC
- 15. Multiple Choice
What is the function of DNA Polymerase?
attach primers to target sequence
separate the DNA
attach nucleotides to the target sequence
separate the fragments according to size
- 16. Multiple Choice
Which of the following best describes the role of primers?Frame the beginning and end of the target sequenceCopy the DNACause DNA strands to separateHeat the sample - 17. Multiple ChoicePCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?11030Over 1 billion
- 18. Multiple ChoiceYou decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.
What PCR "ingredient" would be different in these two tests?Template DNANucleotidesTaq DNA polymerasePrimers - 19. Multiple Choice
How is Fast PCR able to run at such a shorter time frame
Lower the temperature of Denaturation
raises the temperature for annealing so that it and extension can happen almost simultaneously
Shortens the time frame of denatureation
all of the answers are correct
- 20. Multiple Choice
If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?
2
4
8
32
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