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- 1. Multiple Choice
What is the purpose of PCR?
To produce millions of copies of the DNA genome
To produce millions of copies of a specific region of DNA
To add nucleotides to a DNA sequence
To watch polymerase work.
- 2. Multiple Choice
What does PCR stand for?
Polymerase Cytoplasmic Reaction
Polymerase Chromosomal Reaction
Polymerase Chain Reaction
Probably Cannot React
- 3. Multiple Choice
What happens in the denature step of PCR?
The DNA nucleotides are broken apart.
The base-pairing rules for DNA are reversed.
The double-stranded DNA is separated into two single strands of DNA.
The DNA is returned to its natural setting.
- 4. Multiple Choice
At what temperature does the denature step of PCR occur?
94-96° C
50-65° C
72° C
Any temperature
- 5. Multiple Choice
At what temperature does the anneal step of PCR occur?
94-96° C
40-60° C
72° C
Any temperature
- 6. Multiple Choice
At what temperature does the extension step of PCR occur?
94-96°C
50-65°C
72°C
Any temperature
- 7. Multiple Choice
What happens during the anneal step of PCR?
Primers are created.
The primers attach to the target DNA region.
Primers copy the new DNA strand.
Primers sequence DNA
- 8. Multiple Choice
What is the function of a primer?
To identify the particular region of DNA to be copied by PCR.
To copy DNA.
To create DNA nucleotides.
To maintain the temperature of the PCR reaction.
- 9. Multiple Choice
What is the purpose of the extension step of PCR?
To create the primers.
To extend the time it takes to produce DNA.
To allow polymerase to create the complementary strands of DNA.
To allow substrates to copy DNA sequences.
- 10. Multiple Choice
If the target sequence is GCATTA, what is the complementary sequence?
CGUAAU
CGTAAT
CGTUUT
GCATTA
- 11. Multiple Choice
During PCR, the temperature is increased very high. What is the purpose of this?
DNA replication
Strand Separation
Binding Primers
Denaturing the enzymes involved
- 12. Multiple Choice
During PCR, the temperature drops rapidly. What is the purpose of this?
DNA separation
Binding primers
DNA replication
Denaturation
- 13. Multiple Choice
From where does Taq polymerase come?
Bacteria that live in hydrothermal vents/hot springs.
Pig cells
The rain forest
The CDC
- 14. Multiple Choice
Which is NOT as use of PCR?
Cloning
Paternity Testing
Genotyping
DNA Profiling
Protein synthesis
- 15. Multiple Choice
This is a sequence of RNA that binds to DNA to identify the particular region of DNA to be copied by PCR.....
primer
helicase
DNA polymerase
Taq polymerase
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