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- 1. Open EndedWhat is the piece of DNA used for identifying bacteria, and what does this indentification rely on?
- 2. Open EndedDescribe the process of extracting bacterial DNA (sample preparation)
- 3. Open EndedWhy do you heat the sample after adding the digestive buffer?
- 4. Open EndedWhere is the extracted bacterial DNA in the centrifuge tube?
- 5. Open Endedhow do you dissolve the cell wall to extract the bacterial DNA?
- 6. Open Endedwhat is PCR
- 7. Open Endeddescribe what happens in PCR
- 8. Open EndedWhy is the sample cooled after the 95°C water bath?
- 9. Open Endedwhat is used to obtain the desired portion of the DNA in this lab??
- 10. Open Endeddescribe the steps of PCR
- 11. Open Endedwhat is in the PCR Master Mix
- 12. Open Endedwhat is in the positive control
- 13. Open Endedwhat is in the negative control
- 14. Open Endedwhat is the readout for the thermocycler (PCR machine)
- 15. Open Endedwhat are the temperature controls of the thermocycler (PCR machine)
- 16. Open Endedwhat is the purpose of the melt step (95°C for 30s) in the cycle?
- 17. Open Endedwhat is the purpose of the anneal step (60°C 30s) in the cycle?
- 18. Open Endedwhy do the two separated strands of DNA not reanneal during the anneal step?
- 19. Open Endedwhat is the purpose of the extend step (72°C 45s) in the cycle?
- 20. Open Endedafter the final step of PCR purification, what should be in the tube
- 21. Open EndedWhere is the PCR product after you spin the micro concentrator column in a fixed-angle centrifuge?
- 22. Open Endedhow do you loosen the DNA from the column into the collection tube
- 23. Open Endedafter you spin the inverted column at 3000 rpm for 2 minutes, where is the PCR samples
- 24. Open Endedwhats in the final collection tube
- 25. Open Endedwhat is the purpose of inserting a microconcentrator column into the PCR tube?
- 26. Open EndedWhat are dideoxynucleotides?
- 27. Open Endedwhat is the purpose of primers in the sequencing prep
- 28. Open Endedwhat is in the sequencing brews?
- 29. Open Endedwhat are the steps of sequencing prep?
- 30. Open Endedwhat is the purpose of adding distilled water to the purified PCR product
- 31. Open Endedwhat is the point of running the PCR again during the sequencing step
- 32. Open Endeddescribe what is happening in one tube containing the primer 651R during sequencing prep
- 33. Open Endedwhat are in the strip tubes after the PCR is run
- 34. Open Endeddescribe the DNA in each strip tube
- 35. Open Endedhow do you separate the individual DNA pieces and identify the end nucleotide
- 36. Open Endedwhat is gel electrophoresis
- 37. Open Endedexplain what happens in an automatic sequencer
- 38. Fill in the BlankThe smallest piece of DNA that has a fluorescent tag attached to it is the _______.
- 39. Open Endedwhat are the most important tools necessary for sequence matching?
- 40. Open Endedwhat are the BLAST programs
- 41. Open Endedwhat are "conserved" regions
- 42. Open Endedwhat are "variable" regions
- 43. Open Endedwhat is the E-value
- 44. Open Endedwhat is the bacteria in Sample A, and where was the sample obtained from
- 45. Open Endedwhat is the bacteria in Sample B, and where was the sample obtained from
- 46. Open Endedwhat is the bacteria in Sample C, and where was the sample obtained from
- 47. Open Endedwhat is the bacteria in Sample D, and where was the sample obtained from
- 48. Open Endedwhat is the bacteria in Sample E, and where was the sample obtained from
- 49. Open Endedwhat is the bacteria in Sample F, and where was the sample obtained from
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