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Lenses used to change the magnification of the specimen
Body Tube
Eyepiece
Objective lenses
Diaphragm
Moves stage up and down to focus specimen
Stage
Stage clips
Diaphragm
Coarse adjustment
Used to focus specimen, smaller adjustment than coarse adjustment
Fine adjustment
Stage
Arm
Base
Can you use the coarse adjustment on high power?
Yes
No
What power lens should you always start on?
Low
Medium
High
Oil Immersion
is the longer tube marked 40X. Identified by its blue banded color.
Scanner
Low power objective
High power objective
Oil immersion objective (OIO)
same length as the High Power Objective but affording a much higher magnification being stamped 100X. It needs a special oil to be placed on object studied. Identified by its white banded color.
Scanner
Low power objective
High power objective
Oil immersion objective (OIO)
To find total magnification multiply the
Objective lens X the serial number
Objective lens X the Ocular Lens
Ocular Lens X the base number
Ocular lens X the illuminating number
Once oil is used on a slide you should never return to
the oil immersion lens
Scanning power lens
Medium power lens
High dry 40X lens
When you are beginning to focus move the stage to
the lowest position away from the objectives so that you are sure not to break a slide
any position to start, it depends on the size of the specimen
the highest position closest to the low objective
it does not matter where you start focusing
Parfocal
A property of microscopes allowing the specimen to slip out of focus
A property of microscopes allowing the specimen to remain in focus as the viewer moves between magnifications
A property of microscopes allowing the specimen to be focused on with only one objective
The bending of light as it passes from one medium to another for example though the glass of a microscope slide.
retraction
refractive index
immersion oil
refraction
It is okay to get oil on other lens beside the oil immersion lens.
True
False
How should the microscope objectives be positioned when placing the small drop of oil over the specimen?
in between the 40x high dry objective and 100x oil immersion objective.
in between the 100x oil immersion and the scanning objective.
the oil immersion objective should be rotated and clicked into place.
it does not matter as long as you can get the oil under the objective.
When a student finishes using the oil immersion technique they should do which of the following to clean up the microscope.
Unplug the microscope and put the dust cover on.
clean the immersion oil off he high dry 40x lens
clean the immersion oil off the 100x lens, unplug the microscope and put the dust cover on.
don't worry about it the teacher will get it
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