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- 1. Multiple Choice
CRISPR-Cas9 is a type of ________ system found in bacteria.
digestive
endocrine
circulatory
immune
- 2. Multiple Choice
CRISPR-Cas9 was discovered in which of the following organisms?
protists
fungi
bacteria
viruses
- 3. Multiple Choice
Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.
building
editing
destroying
synthesizing
- 4. Multiple Choice
Since the CRISPR-Cas 9 system exists in Bacteria, it can only be used to edit bacterial DNA.
True
False
- 5. Multiple Choice
The enzyme portion of this complex is called ________.
CRISPR
Replicase
Lysozyme
Cas9
- 6. Multiple Choice
What are the two enzymes in a Cas protein complex?
Nuclease
Helicase
Polymerase
Ligase
- 7. Multiple Choice
What does Nuclease do in the Cas protein complex?
cut DNA
open DNA
edit DNA
repair DNA
- 8. Multiple Choice
What type of RNA is used as a GPS of sorts, to find or locate specific genes?
messenger RNA
guide RNA
transfer RNA
ribosomal RNA
- 9. Multiple Choice
In the CRISPR-Cas 9 system, the CRISPR portion is...
a specialized section of DNA
a specialized enzyme used for cutting
Human DNA
guide RNA
- 10. Multiple Choice
What is PAM?
a 3 letter sequence for binding on the target DNA
the main gene portion of the DNA
the cut site for Cas9
a 10 letter sequence for binding on the target DNA
- 11. Multiple Choice
Once the DNA and RNA are bound, Cas9 is activated to cut the DNA ____ upstream from the binding sequence.
1 base
3 bases
6 bases
10 bases
- 12. Multiple Choice
After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following types of repair mechanisms creates a mutation and inactivates the gene.
HDR (homology-directed repair)
"knock out" (NHEJ or non homologous end joining)
- 13. Multiple Choice
After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following repair mechanisms involves "editing" of the gene with insertion of a corrected sequence?
HDR (homology-directed repair)
NHEJ (non-homologous end joining)
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