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- 1. Multiple ChoiceFrom where does Taq polymerase come?Bacteria that live in hydrothermal vents/hot springs.CambodiaThe rain forestThe CDC
- 2. Multiple ChoiceWhy does PCR require a specific type of polymerase?Taq polymerase can withstand the range of temperatures in PCR without denaturing.Taq polymerase is the most inexpensive type of polymerase.Taq polymerase is the most common type of polymerase.A specific type of polymerase is unnecessary.
- 3. Multiple ChoicePCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?11030Over 1 billion
- 4. Multiple Choice
2.1.1 Two abnormal alleles must be inherited for an individual to show symptoms of this type of genetic disorder.
Single gene recessive
Single gene dominant
Single gene sex-linked
mitochondrial
- 5. Multiple Choice
2.1.1 Type of genetic testing that would be most appropriate for parents planning to have children and have a recessive disorder in their family. The parents would like to know the probability that they may pass the disorder on to their future child.
Diagnostic testing
Prenatal testing
Diagnostic testing
Carrier testing (genotyping)
- 6. Multiple Choice
2.1.2 Which of the following is NOT an "ingredient" included in the process of PCR.
Helicase enzyme
Free nucleotides
Primers
taq polymerase enzymes
- 7. Multiple Choice
2.1.2 During which phase of PCR is taq polymerase active?
Denaturation
Annealing
Extension
Cloning
- 8. Multiple Choice
2.1.2 The double helix of DNA is separated using heat during which step of PCR?
Denaturation
Annealing
Extension
Cloning
- 9. Multiple Choice
This karyotype shows aNormal femaleNormal maleDown
Down's Syndrome FemaleDown's Syndrome Male - 10. Multiple Choice
There would appear to be ____ RFLPs in the gel of a person who is a carrier for Cystic Fibrosis.
1
2
3
4
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