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20 questions
Which of these is not an opine
octopine
nopaline
auxin
agropine
Dicarboxy propyl derivative of arginine is
agropine
octopine
nopaline
leucinopine
Isopentyl transferase codes for
tms1
tmr
tms2
tml
Name the phenolic compound secreted from wounded dicot plants
Which of these is an endonuclease?
Vir D2
Vir E2
Vir B
Which of the following is a base plasmid for construction of cosmid
pSC101
pGem3Z
pBR322
LacZ gene
Hybrid vector with ori of plasmid and lambda phage DNA
Cosmid
Phasmid
Phagemid
Which of the following can be used for the synthesis of RNA probes?
lambda ZAP
pBLUESCRIPT
pLFR5
pJB8
Chemically synthesised double stranded DNA molecule with one end blunt and the other end preformed cohesive
Which is the enzyme used for TOPO cloning
Which gene transfer method involves transfer of DNA coated heavy microparticles with high velocity of 300-600 m/s
What is the diameter of Fine capillary needle used in microinjection
Non essential DNA of the phage replaced by foreign DNA in
insertion vector
replacement vector
expression vector
riboprobe vector
Walled intact cells can be penetrated using
electroporation
biolistics
microinjection
In normal vegetative growth which of the vir genes are transcribed
virA
virD
virG
virE
Which portion of the Ti plasmid is transferred to plant nucleus
Which of the following is not a method of selection of recombinants?
Colony hybridization
transformation
nutritional complementation
HAT selection
Missing band is identified in
hybrid arrest translation
hybrid release translation
Hybrid cells are
HGPRT+
HGPRT-
In Spi selection recombinants form plaques. They are Spi-
True
False
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