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- 1. Fill in the Blank
Which type of restriction endonuclease is used in recombinant DNA technology
- 2. Fill in the Blank
The enzymes used to cut and join DNA are
- 3. Multiple Choice
Enzyme that removes a phosphate group from 5' ends of DNA
Acid phosphatase
Lambda exonuclease
Alkaline phosphatase
Terminal transferase
- 4. Multiple Choice
Enzyme that can add homopolymer tails to 3' ends of DNA
Terminal transferase
DNA polymerase
Lambda exonuclease
Polynucleotide kinase
- 5. Fill in the Blank
Name a restriction Endonuclease (no space in between)
- 6. Fill in the Blank
Type II Restriction Endonucleases recognise....
.,....... Sequence
- 7. Multiple Choice
Enzyme that can introduce a small gap of 2 to 3 nucleotides in DNA are
S1 nuclease
Restriction Endonuclease
Lambda exonuclease
- 8. Multiple Choice
Lambda exonuclease cleaved DNA is an excellent substrate for the enzyme
DNA polymerase
Terminal transferase
Polynucleotide kinase
Alkaline phosphatase
- 9. Fill in the Blank
Enzyme that forms a phosphodiester bond between 3'OH of one DNA strand and 5'P of another DNA strand
- 10. Fill in the Blank
A Restriction Endonuclease (without spacing) that creates a 3' overhang
- 11. Multiple Choice
Klenov fragment of DNA polymerase has which of following activities
5' to 3' polymerisation
5' to 3' exonuclease
3' to 5' exonuclease
- 12. Multiple Choice
Enzyme that use ATP and Mg as cofactor and can ligate both DNA and RNA
T4 DNA ligase
E coli DNA ligase
- 13. Multiple Choice
Restriction endonuclease with same sequence specificity and cut site
Isoshizomer
Neoschizomer
Isocaudomer
- 14. Fill in the Blank
Enzyme used in the modification of bases
- 15. Multiple Choice
SmaI and XmaI are
Isoshizomer
Neoschizomer
Isocaudomer
- 16. Fill in the Blank
At high pH if restriction endonuclease cleaves a similar sequence but not identical to their defined recognition sequence is called
- 17. Multiple Choice
Enzyme used to join cohesive ends, blunt ends and linkers
E coli DNA ligase
T4 DNA ligase
Topoisomerase
- 18. Multiple Choice
Enzyme that can phophorylate 5' end of DNA
Terminal transferase
Polynucleotide kinase
Alkaline phosphatase
Lambda exonuclease
- 19. Multiple Choice
An enzyme that is used to identify exons
Lambda exonuclease
S1 nuclease
Terminal transferase
Restriction Endonuclease
- 20. Multiple Choice
Enzyme used in RNA labelling
DNA polymerase
Taq polymerase
RNA polymerase
Topoisomerase
- 21. Multiple Choice
Enzyme with high processivity
E coli DNA polymerase
T4 DNA polymerase
T7 RNA polymerase
Taq polymerase
- 22. Multiple Choice
Enzyme used in PCR
T4 DNA polymerase
T7 DNA polymerase
Taq polymerase
T7 RNA polymerase
- 23. Multiple Choice
Thermophilic polymerase with lowest error rate
Taq
Vent
Pfu
- 24. Multiple Choice
Exo Klenov fragment has
5' to 3' exonuclease activity
3' to 5' exonuclease activity
5' to 3' polymerization activity
All the above
- 25. Fill in the Blank
Enzyme that is used to prevent recirculation of plasmid and dimer formation
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