Enzymes in Recombinant DNA technology
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  • Question 1
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    Q.

    Which type of restriction endonuclease is used in recombinant DNA technology

    answer
    Type II
    alternatives
    Type ii
  • Question 2
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    Q.

    The enzymes used to cut and join DNA are

    answer
    Restriction Endonuclease and Ligases
    alternatives
    Restriction Endonuclease and DNA ligase
    restriction endonuclease, ligase
  • Question 3
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    Q.

    Enzyme that removes a phosphate group from 5' ends of DNA

    answer choices

    Acid phosphatase

    Lambda exonuclease

    Alkaline phosphatase

    Terminal transferase

  • Question 4
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    Q.

    Enzyme that can add homopolymer tails to 3' ends of DNA

    answer choices

    Terminal transferase

    DNA polymerase

    Lambda exonuclease

    Polynucleotide kinase

  • Question 5
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    Q.

    Name a restriction Endonuclease (no space in between)

    answer
    EcoRI
    alternatives
    HindIII
    BamHI
    SmaI
    XmaI
  • Question 6
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    Q.

    Type II Restriction Endonucleases recognise....

    .,....... Sequence

    answer
    Palindrome
    alternatives
    Palindromic
  • Question 7
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    Q.

    Enzyme that can introduce a small gap of 2 to 3 nucleotides in DNA are

    answer choices

    S1 nuclease

    Restriction Endonuclease

    Lambda exonuclease

  • Question 8
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    Q.

    Lambda exonuclease cleaved DNA is an excellent substrate for the enzyme

    answer choices

    DNA polymerase

    Terminal transferase

    Polynucleotide kinase

    Alkaline phosphatase

  • Question 9
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    Q.

    Enzyme that forms a phosphodiester bond between 3'OH of one DNA strand and 5'P of another DNA strand

    answer
    Ligase
    alternatives
    Ligases
    DNA ligase
  • Question 10
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    Q.

    A Restriction Endonuclease (without spacing) that creates a 3' overhang

    answer
    PstI
  • Question 11
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    Q.

    Klenov fragment of DNA polymerase has which of following activities

    answer choices

    5' to 3' polymerisation

    5' to 3' exonuclease

    3' to 5' exonuclease

  • Question 12
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    Q.

    Enzyme that use ATP and Mg as cofactor and can ligate both DNA and RNA

    answer choices

    T4 DNA ligase

    E coli DNA ligase

  • Question 13
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    Q.

    Restriction endonuclease with same sequence specificity and cut site

    answer choices

    Isoshizomer

    Neoschizomer

    Isocaudomer

  • Question 14
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    Q.

    Enzyme used in the modification of bases

    answer
    Methylase
    alternatives
    DNA methylase
  • Question 15
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    Q.

    SmaI and XmaI are

    answer choices

    Isoshizomer

    Neoschizomer

    Isocaudomer

  • Question 16
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    Q.

    At high pH if restriction endonuclease cleaves a similar sequence but not identical to their defined recognition sequence is called

    answer
    Star activity
  • Question 17
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    Q.

    Enzyme used to join cohesive ends, blunt ends and linkers

    answer choices

    E coli DNA ligase

    T4 DNA ligase

    Topoisomerase

  • Question 18
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    Q.

    Enzyme that can phophorylate 5' end of DNA

    answer choices

    Terminal transferase

    Polynucleotide kinase

    Alkaline phosphatase

    Lambda exonuclease

  • Question 19
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    Q.

    An enzyme that is used to identify exons

    answer choices

    Lambda exonuclease

    S1 nuclease

    Terminal transferase

    Restriction Endonuclease

  • Question 20
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    Q.

    Enzyme used in RNA labelling

    answer choices

    DNA polymerase

    Taq polymerase

    RNA polymerase

    Topoisomerase

  • Question 21
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    Q.

    Enzyme with high processivity

    answer choices

    E coli DNA polymerase

    T4 DNA polymerase

    T7 RNA polymerase

    Taq polymerase

  • Question 22
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    Q.

    Enzyme used in PCR

    answer choices

    T4 DNA polymerase

    T7 DNA polymerase

    Taq polymerase

    T7 RNA polymerase

  • Question 23
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    Q.

    Thermophilic polymerase with lowest error rate

    answer choices

    Taq

    Vent

    Pfu

  • Question 24
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    Q.

    Exo Klenov fragment has

    answer choices

    5' to 3' exonuclease activity

    3' to 5' exonuclease activity

    5' to 3' polymerization activity

    All the above

  • Question 25
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    Q.

    Enzyme that is used to prevent recirculation of plasmid and dimer formation

    answer
    Alkaline phosphatase
    alternatives
    alkaline phosphatase
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