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- 1. Multiple Choice
The Meselson-Stahl experiment demonstrated that DNA replication produces two DNA molecules each composed of
Two old strands
Two new strands
One old strand and one new strand
two strands with variable proportions of new and old DNA.
- 2. Multiple Choice
Starting with 15N15N (heavy) DNA, and after TWO generations in the 14N medium, Escherichia coli cells will contain
25% 15N15N DNA, 50% 15N14N DNA, and 25% 14N14N DNA.
50% 15N15N DNA and 50% 14N14N DNA.
50% 15N15N DNA and 50% 15N14N DNA.
50% 15N14N DNA and 50% 14N14N DNA.
25% 15N14N DNA and 75% 14N14N DNA.
- 3. Multiple Choice
Starting with 15N15N (heavy) DNA, and after THREE generations in the 14N medium, E. coli cells will produce the following band(s) in density-gradient centrifugation:
a single band of DNA with medium density.
one band of light DNA and one band of DNA with medium density.
one band of heavy DNA and one band of DNA with medium density.
one band of heavy DNA and one band of light DNA.
three bands corresponding to DNA of light, medium and heavy density.
- 4. Multiple Choice
Meselson and Stahl performed the density-gradient centrifugation of DNA in a solution of calcium chloride.
True
False
- 5. Multiple Choice
The concept that DNA is made of two strands running in opposite directions is called:AntiparallelIsomerismEnantiomerismS/R Chirality - 6. Multiple ChoiceThe two strands of DNA are held together by:Hydrogen BondsPhosphodiester BondsGlycolsyl BondsGlycosidic Linkages
- 7. Multiple ChoiceThe "Double Helix Model" of DNA was proposed by:Watson & CrickMeselson & StahlBeadle & TatumGosling & Watkins
- 8. Multiple ChoiceX-Ray diffraction pictures of DNA that suggested a helical shape were taken by:Watson & CrickMeselson & StahlBeadle & TatumGosling & Franklin
- 9. Multiple ChoiceWho's work concluded that the %A=%T and %G=%CChargaffMeischerLeveneHammerling
- 10. Multiple ChoiceDNA found at the end of each chromosome is called a:TelomereCentromereKinetochoreHistone
- 11. Multiple ChoiceThe new DNA on the leading strand is synthesized in the _________ direction5' to 3'3' to 5'
- 12. Multiple ChoiceDNA replication was proved by the experiments of:Hershey & ChaseMeselson & StahlWatson & CrickFranklin & Gosling
- 13. Multiple ChoiceWhat is the complement to the DNA strand below:
5'-ATGCCAT-3'5'-TACGGTA-3'3'-TACGGTA-5'3'-ATGGCAT-5'5'-ATGGCAT-3' - 14. Multiple ChoiceThe enzyme that joins together small segments of newly replicated DNA is:DNA Polymerase IIIDNA Polymerase IDNA Polymerase IIDNA Ligase
- 15. Multiple Choice
Nucleotides within a single strand of DNA are held together by ____ bonds, while base pairs are joined by ____ bonds.
hydrogen, phosphodiester
phosphodiester, covalent
phosphodiester, hydrogen
phosphodiester, ionic
- 16. Multiple Choice
Which model does NOT predict that after one replication both DNA molecules will be a 50/50 mix of original and new DNA?
conservative
semi-conservative
dispersive
- 17. Multiple Choice
In the Meselson-Stahl experiment, E.coli was transfered from 15N medium to 14N medium. Centrifugation of DNA after 20 minutes in 14N produced ____ and disproved the ____ model.
1 intermediate band, conservative
1 intermediate band, dispersive
1 heavy band and 1 light band, semi-conservative
1 light and 1 intermediate band, dispersive
- 18. Multiple Choice
In the Meselson-Stahl experiment, E.coli was transfered into 14N medium from 15N medium. The bacteria is incubated and reproduced up to 2 generation of offsprings. Centrifugation of DNA after 40 minutes in 14N produced ____ and disproved the ____ model.
1 light and 1 intermediate band, dispersive
1 light and 1 intermediate band, conservative
1 intermediate band, dispersive
1 heavy and 1 light band, semi-conservative
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