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- 1. Multiple Choice
What is transformation?
The use of viruses to transform or genetically engineer cells.
The measure of how well cells are transformed in a new phenotype.
The insertion of a plasmid with a foreign gene into a bacterial cell which results in new acquired traits.
Genetic engineering or transformation of mammalian cells.
- 2. Multiple Choice
When fragments of DNA are connected from different sources, this creates:
A plasmid
A clone
Recombinant DNA
Fragmented DNA
- 3. Multiple Choice
Bacterial DNA that is often circular or in the shape of a ring is called a:
Clone
Plasmid
Fragment
Recombinant DNA
- 4. Multiple Choice
Organisms that have had DNA from other organisms inserted into their DNA to produce certain traits are called:
Plasmids
Clones
Transgenic organisms
Recombinant organisms
- 5. Multiple Choice
Which of the following is a medical application of genetic engineering?
Inserting human DNA into bacterial DNA for insulin production
Manipulating of DNA to produce glowing monkeys
Engineering crops for greater nutritional content
Modifying genes to achieve a desirable phenotype
- 6. Multiple Choice
Which of the following means of genetic engineering would have the greatest impact on world hunger?
Inserting human DNA into bacterial DNA for insulin production
Manipulating DNA in trees to require less water
Engineering crops for greater nutritional content
Modifying genes to achieve a desirable phenotype
- 7. Multiple Choice
Inserting human DNA into a bacterial plasmid to create insulin-producing bacteria is an example of:
Recombinant DNA
Bacterial Transformation
Cloning
Selective breeding
- 8. Multiple Choice
Crops are often genetically engineered for improved traits. Such crops and organisms are called:
Genetically modified organisms
Clones
Transformed
Nutritionally improved
- 9. Multiple Choice
DNA can be cut with
ligase
helicase
restriction enzymes
DNA scissors
- 10. Multiple Choice
A scientist wants to combine a bacterial plasmid with a human insulin gene.
She should use the same restriction enzyme to cut them to make blunt ends
She should use the same restriction enzyme to cut them to make sticky ends
She should use different restriction enzyme to cut them to make blunt ends
She should use different restriction enzyme to cut them to make sticky ends
- 11. Multiple Choice
What are the steps of bacterial transformation in order?
cut insulin gene and plasmid with same restriction enzyme, wait for insulin gene to start making insulin, put the plasmid into the bacteria cell
cut insulin gene and plasmid with same restriction enzyme,isolate insulin gene, , combine insulin gene and plasmid at sticky ends to make a recombinant plasmid, insert the recombinant plasmid into a bacteria cell
isolate insulin gene, cut insulin gene and plasmid with same restriction enzyme, combine insulin gene and plasmid at sticky ends to make a recombinant plasmid, insert the recombinant plasmid into a bacteria cell
isolate plasmid, cut insulin gene and plasmid with same restriction enzyme, wait for insulin gene to start making isulin,combine insulin gene and plasmid at sticky ends to make a recombinant plasmid
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