Have an account?
Skip to Content
Build your own quiz
Suggestions for you
17 questions
Show Answers
See Preview
- 1. Multiple Choice
What is pGlo?
A plasmid that dies in the presence of ampicillin
A gene for green fluorescent protein (GFP)
A plasmid with genes for GFP and ampicillin resistance
A chemical that makes jelleyfish glow
- 2. Multiple Choice
The purpose of the Petri dish with the p-Glo plasmid added and ampicillin is to...
Fight off invading bacteria
Identify transformed bacteria
Kill all bacteria so the dish is sterile
Destroy extra plasmids
- 3. Multiple Choice
Why do you not add plasmid to the -pGlo tube?
The plasmid was already added to the -pGlo tube before
Instructions are wrong; you should add the plasmid in to the - tube
The -pGlo tube is a control, with bacteria but no plasmid
- 4. Multiple Choice
On which plate would you find bacteria most like the original non-transformed E. coli?
-pGlo, -AMP
-pGlo, + AMP
+pGlo, +AMP
- 5. Multiple Choice
What is the point of the heat shock and transformation solution?
To kill bacteria we don't want
To make the bacteria's membrane open so the pGlo can go in
To heat the plasmid up so ligase will work
To make the pGlo plasmid
- 6. Multiple Choice
How often do you need a new sterile loop?
You'll use the same one the entire lab
You should switch every 5 minutes
Use a new loop every time you touch new/different bacteria/plasmid
It doesn't matter
- 7. Multiple Choice
How many times do you put the tubes on ice, and for how long?
Once for 5 min
Twice for 5 min each
Once for 10 min, a second time for 2 min
5 min, then 2 min, then 5 min again
- 8. Multiple Choice
How long should the tubes be in the 42oC water bath?
30 seconds
50 seconds
60 seconds
120 seconds
- 9. Multiple Choice
How many of your plates will have the -pGlo bacteria on them?
1
2
3
4
- 10. Multiple Choice
On which plate would you find transformed E. coli?
-pGlo, -AMP
-pGlo, + AMP
+pGlo, +AMP
- 11. Multiple Choice
On which plate would you find glowing transformed E. coli?
-pGlo, -AMP
-pGlo, + AMP
+pGlo, +AMP
+pGlo, +AMP + ara
- 12. Multiple Choice
On which plate would you find NOTHING growing?
-pGlo, -AMP
-pGlo, + AMP
+pGlo, +AMP
+pGlo, +AMP + ara
- 13. Multiple Choice
What is LB?
lab
Label B
broth / food
antibiotics
- 14. Multiple Choice
What is Amp?
voltage unit
to be hyper
ampicillin
amplitutde
- 15. Multiple Choice
What is Ara?
arabinose sugar
arachnids
arabic
a clone
- 16. Multiple Choice
What is used as a transformation solution?
water
antibiotics
calcium chloride
luria broth
- 17. Multiple Choice
What is the name of this type of procedure?
gel electrophoresis
bacterial transformation
cloning
biotech
- Answer choicesTagsAnswer choicesTags