pGlo lab pre-test
Assessment
•
Brenna Raugewitz
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Biology
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9th - 12th Grade
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65 plays
•
Medium
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17 questions
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1.
Multiple Choice
What is pGlo?
A plasmid that dies in the presence of ampicillin
A gene for green fluorescent protein (GFP)
A plasmid with genes for GFP and ampicillin resistance
A chemical that makes jelleyfish glow
2.
Multiple Choice
The purpose of the Petri dish with the p-Glo plasmid added and ampicillin is to...
Fight off invading bacteria
Identify transformed bacteria
Kill all bacteria so the dish is sterile
Destroy extra plasmids
3.
Multiple Choice
Why do you not add plasmid to the -pGlo tube?
The plasmid was already added to the -pGlo tube before
Instructions are wrong; you should add the plasmid in to the - tube
The -pGlo tube is a control, with bacteria but no plasmid
4.
Multiple Choice
On which plate would you find bacteria most like the original non-transformed E. coli?
-pGlo, -AMP
-pGlo, + AMP
+pGlo, +AMP
5.
Multiple Choice
What is the point of the heat shock and transformation solution?
To kill bacteria we don't want
To make the bacteria's membrane open so the pGlo can go in
To heat the plasmid up so ligase will work
To make the pGlo plasmid
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