HISTOPATH: EMBEDDING

1.

Multiple Select

30 sec

1 pt

Is the process by which impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is then allowed to solidify

Embedding

Casting

Blocking

2.

Multiple Choice

30 sec

1 pt

The melting point of the wax

5-10℃ above

5-10℃ below

3.

Multiple Select

30 sec

1 pt

Cooled rapidly in a refrigerator at

-5℃

Immersed in cold water

4.

Multiple Select

30 sec

1 pt

Orientation: process by which a tissue is arranged in precise positions in the mold during embedding, on the microtome before cutting, and on the slide before staining

TRUE

FALSE

5.

Multiple Choice

30 sec

1 pt

Consists of 2 L-shaped strips of heavy brass or metal arranged on a flat metal plate ▪ can be moved to adjust the size of the mold to the size of the specimen ▪ recommended for routine use

Leuckhart’s Embedding Mold

Compound Embedding Unit

Plastic Embedding Rings and Base Mold

Disposable Embedding Molds

6.

Multiple Choice

30 sec

1 pt

Made up of a series of interlocking plates resting on a flat metal base ▪ has the advantage of embedding more specimens at a time

Leuckhart’s Embedding Mold

Compound Embedding Unit

Plastic Embedding Rings and Base Mold

Disposable Embedding Molds

7.

Multiple Choice

30 sec

1 pt

Consists of a special stainless steel base mold fitted with a plastic embedding ring

Leuckhart’s Embedding Mold

Compound Embedding Unit

Plastic Embedding Rings and Base Mold

Disposable Embedding Molds

8.

Multiple Choice

30 sec

1 pt

A. Peel-Away disposable thin plastic embedding molds - B. Plastic Ice Trays: recommended for busy routine laboratories - C. Paper Boats

Leuckhart’s Embedding Mold

Compound Embedding Unit

Plastic Embedding Rings and Base Mold

Disposable Embedding Molds

9.

Multiple Choice

30 sec

1 pt

Equipped with a warm plate and a cold plate at -5℃

Tissue Tek

Tissue Fek

10.

Multiple Select

30 sec

1 pt

Tissue Tek Advantages

ease of use

less paraffin wax needed

faster embedding

firmly attached tissue and holder and permanent identification

11.

Multiple Choice

30 sec

1 pt

Embedded molds should bear the case number and other identification data of the tissue block

True

False

12.

Multiple Choice

30 sec

1 pt

Recommended for embedding hard tissues and for large sections of whole organs ▪ Bell jars were used to control the rate of evaporation of the solvent

Celloidin or Nitrocellulose Method

Double-Embedding Method

13.

Multiple Choice

30 sec

1 pt

The process in which tissues are first infiltrated with celloidin and subsequently embedded in paraffin mass ▪ used to facilitate cutting of large blocks of dense firm tissues ▪ recommended for making small sections of celloidin blocks

Celloidin or Nitrocellulose Method

Double-Embedding Method

14.

Multiple Choice

30 sec

1 pt

Superior results for light microscopic studies (hard tissues such as undecalcified tissues) ▪ for high resolution light microscopy of tissue sections thinner than the usual 4-6 um

Plastic (Resin) Embedding

Solid Embedding

15.

Multiple Choice

30 sec

1 pt

Balanced mixture of epoxy plastic, catalysts, and accelerators; Vinylcyclohexane dioxide (VCD), one of its components, is carcinogenic

Epoxy embedding plastics

Polyester plastics

Acrylic plastics

16.

Multiple Choice

30 sec

1 pt

Introduced for electron microscopy

Epoxy embedding plastics

Polyester plastics

Acrylic plastics

17.

Multiple Choice

30 sec

1 pt

Made up of esters of acrylic or methacrylic acid ▪ used extensively for light microscopy

Epoxy embedding plastics

Polyester plastics

Acrylic plastics

18.

Multiple Choice

30 sec

1 pt

Is added to the plastic as a catalyst that decomposes to form phenyl radicals acting as active sites for the polymerization of acrylics

Benzoyl peroxide

Hydrogen peroxide:

19.

Multiple Choice

30 sec

1 pt

Epoxy Plastic: Slow

Bisphenol A (Araldite)

Glycerol (Epon)

Cyclohexene dioxide (Spurr)

20.

Multiple Choice

30 sec

1 pt

Epoxy Plastic: Lower viscosity - Often sold as mixtures of isomers

Bisphenol A (Araldite)

Glycerol (Epon)

Cyclohexene dioxide (Spurr)

21.

Multiple Choice

30 sec

1 pt

Epoxy Plastic: Very low viscosity - Infiltrate fastest

Bisphenol A (Araldite)

Glycerol (Epon)

Cyclohexene dioxide (Spurr)

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