Is the process by which impregnated tissue is placed into a precisely arranged position in a mold
containing a medium which is then allowed to solidify
Embedding
Casting
Blocking
2. Multiple Choice
30 seconds
1 pt
The melting point of the wax
5-10℃ above
5-10℃ below
3. Multiple Choice
30 seconds
1 pt
Cooled rapidly in a refrigerator at
-5℃
Immersed in cold water
4. Multiple Choice
30 seconds
1 pt
Orientation: process by which a tissue is arranged in precise positions in the mold during embedding, on the microtome before cutting, and on the slide before staining
TRUE
FALSE
5. Multiple Choice
30 seconds
1 pt
Consists of 2 L-shaped strips of heavy brass or metal arranged on a flat metal plate ▪ can be moved to adjust the size of the mold to the size of the specimen ▪ recommended for routine use
Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings and Base Mold
Disposable Embedding Molds
6. Multiple Choice
30 seconds
1 pt
Made up of a series of interlocking plates resting on a flat metal base ▪ has the advantage of embedding more specimens at a time
Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings and Base Mold
Disposable Embedding Molds
7. Multiple Choice
30 seconds
1 pt
Consists of a special stainless steel base mold fitted with a plastic embedding ring
Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings and Base Mold
Disposable Embedding Molds
8. Multiple Choice
30 seconds
1 pt
A. Peel-Away disposable thin plastic embedding molds - B. Plastic Ice Trays: recommended for busy routine laboratories - C. Paper Boats
Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings and Base Mold
Disposable Embedding Molds
9. Multiple Choice
30 seconds
1 pt
Equipped with a warm plate and a cold plate at -5℃
Tissue Tek
Tissue Fek
10. Multiple Choice
30 seconds
1 pt
Tissue Tek Advantages
ease of use
less paraffin wax needed
faster embedding
firmly attached tissue and holder and permanent identification
11. Multiple Choice
30 seconds
1 pt
Embedded molds should bear the case number and other identification data of the tissue block
True
False
12. Multiple Choice
30 seconds
1 pt
Recommended for embedding hard tissues and for large sections of whole organs ▪ Bell jars were used to control the rate of evaporation of the solvent
Celloidin or Nitrocellulose Method
Double-Embedding Method
13. Multiple Choice
30 seconds
1 pt
The process in which tissues are first infiltrated with celloidin and subsequently embedded in paraffin mass ▪ used to facilitate cutting of large blocks of dense firm tissues ▪ recommended for making small sections of celloidin blocks
Celloidin or Nitrocellulose Method
Double-Embedding Method
14. Multiple Choice
30 seconds
1 pt
Superior results for light microscopic studies (hard tissues such as undecalcified tissues) ▪ for high resolution light microscopy of tissue sections thinner than the usual 4-6 um
Plastic (Resin) Embedding
Solid Embedding
15. Multiple Choice
30 seconds
1 pt
Balanced mixture of epoxy plastic, catalysts, and accelerators; Vinylcyclohexane dioxide (VCD), one of its components, is carcinogenic
Epoxy embedding plastics
Polyester plastics
Acrylic plastics
16. Multiple Choice
30 seconds
1 pt
Introduced for electron microscopy
Epoxy embedding plastics
Polyester plastics
Acrylic plastics
17. Multiple Choice
30 seconds
1 pt
Made up of esters of acrylic or methacrylic acid
▪ used extensively for light microscopy
Epoxy embedding plastics
Polyester plastics
Acrylic plastics
18. Multiple Choice
30 seconds
1 pt
Is added to the plastic as a catalyst that decomposes to form phenyl radicals acting as active sites for the polymerization of acrylics
Benzoyl peroxide
Hydrogen peroxide:
19. Multiple Choice
30 seconds
1 pt
Epoxy Plastic: Slow
Bisphenol A (Araldite)
Glycerol (Epon)
Cyclohexene dioxide (Spurr)
20. Multiple Choice
30 seconds
1 pt
Epoxy Plastic: Lower viscosity - Often sold as mixtures of isomers
Bisphenol A (Araldite)
Glycerol (Epon)
Cyclohexene dioxide (Spurr)
21. Multiple Choice
30 seconds
1 pt
Epoxy Plastic: Very low viscosity - Infiltrate fastest