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- 1. Multiple Choice
What category of medical intervention would vitamins, vaccines, and helmets fall under
Treatment
Prevention
Diagnosis
Therapeutic
- 2. Multiple Choice
When determining the genetic sequence of an organism; PCR is used to duplicate DNA, enzymes cut it into different sized pieces, electrophoresis is used to sort it and laser is used to
read the genetic sequence based on fluorescent nucleotides
cut the DNA fragments into individual genes segments
calculate the speed at which the DNA fragments move through the gel
All of these
- 3. Multiple Choice
While working in a pathogen lab an epidemiologist asks you to run a BLAST, what are they expecting you to do?
Search a national database to match the genome sequence of the sample
Use restriction enzymes to cut DNA fragments into smaller and smaller pieces
Use hot vapor to disinfect a room contaminated by an unknown pathogen
Determine what anitbodies are needed to deactivate a specific antigen
- 4. Multiple Choice
The BLAST database is hosted on the NCBI National Center for Biotechnology Information, what type of information might they also be able to provide
List of symptoms of all known illnesses
DNA and RNA sequences of different viruses
Samples of different bacterial cultures
Genetically modified laboratory organisms
- 5. Multiple Choice
What does it mean to 'amplify' a DNA sample
Recombine DNA using restriction enzymes
Grow bacteria that host that DNA fragment in a petri dish
Use PCR to make many more copies
Run the DNA fragment through a sequence to get the genome
- 6. Multiple Choice
B cells produce uniquely shaped proteins called antibodies that bind to ______ to mark them for destruction.
Enzymes
Secondary Antibodies
Antigens
Hormones
- 7. Multiple Choice
When using an ELISA test to determine if a patient tests positive for a disease a fluid sample from the patient is used. If the patient is positive for the disease the _____ will bind to the test well first.
Antibody
Antigen
Pathogen
Secondary Antibody
- 8. Multiple Choice
What is the role of the substrate in an ELISA test?
It binds directly to the antigen if the antigen is present
It washes excess fluid away from the antibodies to the antigen can bind
It binds to the secondary antibody and causes a color change
No substrate is used in an ELISA
- 9. Multiple Choice
Which is the correct sequence when performing an ELISA
substrate > antigen > primary antibody > secondary antibody > enzyme
primary antibody > secondary antibody >substrate > antigen > enzyme
secondary antibody >substrate > antigen > primary antibody > enzyme
antigen > primary antibody > secondary antibody > enzyme > substrate
- 10. Multiple Choice
The results of ELISA may vary based on concentration of
antigen present
secondary antibody
primary antibody
substrate
- 11. Multiple Choice
This ELISA samples show different tints of blue. What can we infer about the patients who's test resulted in light tinted blues?
They are likely sickest because this shows their body isn't making many antibodies
They are probably getting over being sick because the light color indicates they have very few antigens
We can ONLY determine that they have a lower number of antigens
We can ONLY determine that they have the most antibody activity
- 12. Multiple Choice
In and ELISA test the secondary antibody is specially created in a lab to have an enzyme attached to it. What is the role of that enzyme
The enzyme binds to the antigen to inactivate it and keep the lab safe
The enzyme causes the color change in the substrate
The enzyme helps wash antigens out of the test
The enzyme binds the secondary antibody to the primary antibody
- 13. Multiple Choice
What category of medical intervention would an antibiotic most likely fall into?
Prevention
Treatment
Diagnostic
Bioinformatics
- 14. Multiple Choice
What information is needed to best use the BLAST bioinformatics tool?
The symptoms of the disease
The number of people infected
The concentration of the the ELISA results
A nucleotide sequence
- 15. Multiple Choice
When developing a genome a DNA stand is cut into fragments that vary by 1 nucleotide in length. How is this DNA then sorted?
A centrifuge spins the DNA and the smallest pieces float to the top and the larger sink to the bottom
A laser is used to read the different amounts of fluorescent nucleotides
Gel electrophoresis is used with the smallest piece of DNA traveling the fastest through the gel
Computer simulations are used to capture the amount of light refracted from the DNA segments
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