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- 1. Multiple Choice
Which amino acid has three pKa values?
leucine
proline
glycine
threonine
cysteine
- 2. Multiple Choice
Which statement is correct about peptides?
Peptides have no α-carboxyl groups.
Peptides have their amino acid sequences
written from the N-terminus.
Peptides do not have isoelectric points.
Peptides are not biologically active.
Peptide bonds are broken through
condensation reactions.
- 3. Multiple Choice
A new protein resembling myosin was reported. Unlike myosin, it binds calcium. Its isoelectric point and molecular weight are very similar to those of myosin. Which method would BEST separate the new protein from myosin if those two proteins were in the same buffer solution?
ion-exchange chromatography
size-exclusion chromatography
affinity chromatography
dialysis
fractionation
- 4. Multiple Choice
What measurement increases during purification of an enzyme?
activity
total protein
specific activity
fraction volume
- 5. Multiple Choice
The amino acid sequences of proteins:
can be used to establish evolutionary relationships
between organisms.
are an example of tertiary structure.
are similar within a given organism.
are also referred to as “consensus sequences.”
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