Kary Mullis developed PCR in 1983.

At what temperature is the annealing step in PCR carried out?

Application of PCR includes genotyping, cloning, mutation detection, sequencing, microarrays, forensic and paternity test.

Small amount of DNA is NOT required per test.

PCR is less precise in determining the sizes of alleles.

STATE THE CORRECT ANSWER. Following the last cycle, the enzyme is allowed to finish any incomplete synthesis by including a final extension at 65°C for 2 mins

In the laboratory, PCR is performed in controlled conditions.

PCR is usually carried out by those who have graduated from the following courses, EXCEPT:

The purpose of PCR is to amplify a lot of double stranded DNA molecule with the different size and sequence by enzymatic method and cycling.

The following are components of PCR:

They expand the growing DNA strand with the help of Taq polymerase