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10 questions
The first genomic libraries were cloned in _______________
Plasmid
Bacteria
Human
Plants
Genomic library construction is concerned with ___________
Gene isolation
Protein production
Antibiotics
Regeneration
A genomic library is a collection of _____________
Genes
Proteins
Vectors
Recombinants
The Klenow fragment is basically a
DNA hybrid
DNA polymerase
RNA polymerase
Promoter
Which organism has the highest number of vectors?
Yeast
Mammalian cells
E.coli
Fungi
Which is an example of a simplest vector (in terms of size)?
2 micron circle
Bacteriophage
Plasmid
YAC
Which antibiotic resistance is present in pBR322?
Ampicillin
Kanamycin
Lactase
Gentamycin
Chromosomal or genomic DNA is separated by
Sedimentation
Dissolution in water
Centrifugation
Distillation
Proteins can be removed via treatment by?
Treatment with sodium hydroxide
Phenol and chloroform treatment
Chloroform treatment alone
Centrifuging
Cell lysis is carried out by which substance?
Water
Lysozyme and detergents
Suphuric Acid
Sugar solution