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- 1. Multiple Choice
Protoplasts are__________
Cell without a nucleus
Cell without a Cell wall
Cell without a Plasma membrane
Cell without a genetic material
- 2. Multiple Choice
__________ is the first step for protoplast culture
Selection of Explant
Viability testing for protoplast
Isolation of protoplast
Preparation of culture media
- 3. Multiple Choice
Cellulase enzyme in isolation of protoplast is used ________
To degrade proteins
To degrade cellulose
To degrade pectin
To degrade hemicellulose
- 4. Multiple Choice
The production of hybrid plants through fusion of two different somatic protoplasts is known as
Protoplast hybrids
Protoplast fusion
Somatic hybridization
Somatic hybrids
- 5. Multiple Choice
1st report on protoplast fusion-derived somatic hybrid plant was reported by
Carlson et al
Hanstein
Klercker
Cocking
- 6. Multiple Choice
The word “PROTOPLAST” was coined by
Hanstein
Klercker
Cocking
Tkebe et al
- 7. Multiple Choice
Isolation of protoplasts mechanically from higher plants was pioneered by
Klercher
Tkebe et al
Cocking
Both A & B
- 8. Multiple Choice
The principle deficiency of mechanical method of isolation is _________
protoplasts released are few in number.
protoplasts are damaged
Viability of protoplasts is high
All of the above
- 9. Multiple Choice
The most commonly used pectinase is
Macerozyme
Rhozyme HP 150
Cellulase
Pectiolose
- 10. Multiple Choice
Friable tissues are having _________
low starch content generally yields better results
high starch content generally yields better results
low sucrose content generally yields better results
low sucrose content generally yields better results
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