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39 questions
Negative appearance for reduction?
no color present
color present
milky purple
milky pink
Positive appearance for reduction?
milky pink
milky purple
no color present
color present
How do you know if CO2 has been produced in your litmus test?
bubbles are present
cracks in the curd
softening of curd
no bubble present
The negative appearance of fermentation, alkalinization, and peptonization?
blueberry milk
pink milk
purple milk
lack of color
Positive appearance of reduction for a BASE?
white milk
colorless
apple juice color
dark blue
Positive appearance of reduction for an ACID?
white milk
purple juice
pink milk
apple juice
Positive appearance of peptonization?
bleaching
pink milk
Grape and apple juice layers (translucent)
translucent with pink layers
Positive appearance of Alkalinization?
blueberry milk
pink milk
grape juice
pink milk
Positive appearance of Fermentation?
purple milk
blueberry milk +/- hard curd
pink milk +/- hard curd
pink milk +/- Rennet curd
What is the end product of curd formation?
cracks
curd
leucolitmus
ammonia
What are the end products of litmus reduction?
polypeptides and amines
Amino Acids
Leucolitmus (white/clear) and ATP
ATP + Organic Acids +/- CO2 gas
What are the end products of peptonization?
Reduced Litmus + ATP
Amino Acids and Ammonia
Peptides and Ammonia
ATP and Organic Acids
What are the end products of alkalinization?
polypeptides + acids
ATP + Organic Acids
Reduced Litmus + ATP
polypeptides + Basic Amines
What are the end products of fermentation?
only CO2
polypeptides + amino acids
ATP + amino acids
Organic acid, ATP, and carbon dioxide
Curd formation is a result of either of which two pathways?
alkalinization and fermentation
peptonization and reduction
fermentation or peptonization
reduction and fermentation
What pathway does reduction test for?
ATP
ETP
Organic Acids
Amino Acids
What enzymes do alkalinization and peptonization test for?
proteases
fermentation
reduction
ETP
What pathway does fermentation test for?
proteases
peptonization
ETP
fermentation
What is the medium for all the litmus milk reactions?
litmus milk
Carb Broth
Basic Amines
casein
What is the "substrate" of curd formation?
casein
casein and lactalbumin
litmus
Acidic or basic conditions
What is the substrate of reduction?
litmus
casein
casein + lactalbumin
lactose
What is the substrate of alkalinization?
Casein
casein and lactalbumin
litmus
lactose
What is the substrate of peptonization?
casein
casein and lactalbumin
lactose
litmus
What is the substrate of fermentation?
lactalbumin
litmus
casein
lactose
Which tubes have most likely formed curds?
1, 2, 3
1, 3, 5
1, 2, 4
2, 3, 5
Which tube has undergone partial hydrolysis of casein?
tube 5
tube 1
tube 2
tube 4
Which tubes show signs of reduction?
tubes 1 and 3
tubes 2 and 3
tubes 2 and 4
tubes 4 and 5
Which tubes have acidic conditions?
tubes 2 and 4
tubes 1 and 2
tubes 1 and 3
tubes 4 and 5
Which tubes have basic/alkaline conditions?
tubes 1 and 2
tubes 3 and 4
tubes 1 and 5
tubes 2 and 4
What reactions have taken place in this tube? Is the tube acidic or basic?
fermentation, alkalinization, acidic
peptonization, reduction, basic
peptonization, reduction, acidic
fermentation, alkalinization, basic
What reactions have taken place in this tube? (Note that the medium is now solid.)
fermentation, reduction, curd formation
alkalinization, reduction, curd formation
peptonization, reduction, curd formation
peptonization, alkalinization, curd formation
Identify the tube that has undergone fermentation
tube 4
tube 3
tube 1
tube 3
Identify the tube that has undergone reduction
tube 1
tube 2
tube 3
tube 4
Identify the tube that has undergone peptonization
tube 4
tube 1
tube 3
tube 2
Identify the tube that has undergone alkalinization
tube 1
tube 4
tube 3
tube 2
What is alkalinization?
complete hydrolysis of a protein
incomplete hydrolysis of a protein
litmus being reduced
Lactose metabolized to form acid products
What is fermentation?
Lactose metabolized to form acid products
Complete hydrolysis of protein
incomplete hydrolysis of protein
What is peptonization?
Complete hydrolysis of protein
incomplete hydrolysis of protein
Litmus reduced to leucolitmus
Lactose metabolized to form acid products
What is reduction?
Lactose metabolized to form acid products
complete hydrolysis of a protein
incomplete hydrolysis of a protein
Litmus reduced to leucolitmus
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