Heat the inoculating loop

Open the bacteria bottle

Take the lid off the petri dish

Wipe surfaces with disinfectant

To get as many pathogens as possible in the petri dish

To transfer only the wanted bacteria onto the petri dish

To kill as many bacteria as possible

To get a variety of different microorganisms in the culture

$25\degree$

$35\degree$

$45\degree$

$55\degree$

$15\degree C$

$30\degree C$

$25\degree C$

$40\degree C$

Only one colour would be seen on the plate

More than one colour would be on the plate

Furry fungi could be seen

The plate smells bad

so no other harmful bacteria form

so the conditions do not become anaerobic

to save the school money on tape

To save money on powering the autoclave

to stop from melting the petri dish

So that only non pathogenic bacteria are cultured

To prevent damage to the agar