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- 1. Multiple Choice
Beer’s law states that
Absorbance is inversely proportional to concentration of solution and pathlength
Absorbance is directly proportional to concentration of solution and pathlength
Absorbance is inversely proportion to concentration of solution and length of instrument
None of the above
- 2. Multiple Choice
Deviation of Beer’s law occurs when
Intensity of colour is not very dark
Polychromatic light is used
Monochromatic light is used
Both a and b
- 3. Multiple Choice
Chromophore means
A part of molecule responsible for its colour
A moiety which undergoes conformational changes when hit by light
A part of molecule responsible for the intensity
Both a and b
- 4. Multiple Choice
Molar extiction coefficient can be determined by formula (A-absorbance, ε-molar extinction coefficient, C-concentration, L-pathlength)
A = ε * C * L
ε = A * C * L
ε = (A * C) / L
ε = A / (C * L)
- 5. Multiple Choice
All are applications of UV-visible spectrophotometer except
To determine the unknown compounds depending on spectrum
To determine the functional groups
To determine the temperature
To determine the concentration of DNA
- 6. Multiple Choice
Colorimeter is
The instrument which measures absorbance at particular wavelength of light
The instrument which measures the intensity of colour solution
The instrument which measure the temperature
Both a and b
- 7. Multiple Choice
Major application of IR spectroscopy is to determine the functional group
True
False
- 8. Multiple Choice
Thin layer chromatography is
partition chromatography
electrical mobility of ionic species
adsorption chromatography
none of the above
- 9. Multiple Choice
Which of the following is not a highly specific biological interaction used in affinity chromatography?
Cations-anions
Antigen-antibody
Enzyme-substrate
Receptor-ligand
- 10. Multiple Choice
Identify the resin used in anion-exchange chromatography?
Diaethylaminoethyl
Carboxymethyl cellulose
Quaternary amine
none of the above
- 11. Multiple Choice
Gas chromatography can be performed in which of the following ways?
Only in columns
Only on plane surfaces
Either in columns or on plane surfaces
Neither in columns nor on plane surfaces
- 12. Multiple Choice
Characteristic feature of any form of chromatography is
Use of molecules that are soluble in water
Use of inert carrier gas
Calculation of Rf values for the molecules separated
Use of mobile and stationary phase
- 13. Multiple Choice
Chromatography is a physical method that is used to separate and analyse
Simple mixtures
Complex mixtures
Viscous mixtures
Metals
- 14. Multiple Choice
In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?
Column chromatography
Planar chromatography
Liquid chromatography
Gas chromatography
- 15. Multiple Choice
In chromatography, which of the following can the mobile phase be made of?
Solid or liquid
Liquid or gas
Gas only
Liquid only
- 16. Multiple Choice
Which of the following cannot be used as an adsorbent in Column adsorption chromatography
Magnesium oxide
Silica gel
Activated alumina
Potassium permanganate
- 17. Multiple Choice
What is the Analyte?
Substance for separation
Substance for impurity
A and B
None of the above
- 18. Multiple Choice
When is electrophoresis not used?
Separation of proteins
Separation of amino acids
Separation of lipids
Separation of nucleic acid
- 19. Multiple Choice
Which membrane are used in western blotting
Filter paper
Nitrocellulose membrane
Cellulose acetate
None of the above
- 20. Multiple Choice
The neutral atoms of all isotopes of the same element contain the same number of
neutrons only
electrons
mass numbers
masses
- 21. Multiple Choice
The atomic number is not changed by which type of radioactive decay?
Beta
Gamma
Alpha
The atomic number is affected by all forms of radioactive decay
- 22. Multiple Choice
What is half-life in radioactivity
It is half the time for radioactive sources to become safe
It is half the time for an atom to decay
it is half the time it takes the activity of the source to decrease to zero
It is time which takes to make the activity of the source to decrease to half
- 23. Multiple Choice
What are the units of radioactivity?
Becquerel and curie
Kcal and cal
Kpascal and pascal
None of the above
- 24. Multiple Choice
In isopycnic centrifugation only particle size only affects the rate at which particles move until their density is the same as the surrounding gradient medium
True
False
- 25. Multiple Choice
Which of these is a types of ultracentrifuge?
Preparative and analytical
Isopycnic and rate-zonal
Differential and ultracentrifugation
None of the above
- 26. Multiple Choice
Radioactive labelling examples are
Tritium
Carbon14
Sodium22
All the above
- 27. Multiple Choice
The number of protons or atomic number is reduced to 2 by which form of radioactive decay
Beta decay
Gamma decay
Alpha decay
None of the above
- 28. Multiple Choice
Factors affecting sedimentation velocity
V α d2
V α (ρs- ρs)
Both a and b
None of the above
- 29. Multiple Choice
Thin layer chromatography is
partition chromatography
electrical mobility of ionic species
adsorption chromatography
none of the above
- 30. Multiple Choice
Partition principle is
Solute equilibrates between stationary and mobile phase
Solvent equilibrates between stationary and mobile phase
Both a and b
None of the above
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