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- 1. Multiple Choice
During PCR , DNA sample is cooled to 54 0C, allowing primers attach to opposite ends of the target sequence. That step in the PCR is called:
Denaturation
Annealing
Elongation
Condensation
- 2. Multiple Choice
Genetic modification is carried out by gene transfer between species. The name of the enzyme that joins the sticky ends , fixing the gene into the plasmid is :
Restriction enzyme
DNA Ligase
Recombinant plasmid enzyme
Vector
- 3. Multiple Choice
Define clone:
A group of genetically-identical organisms derived from a single original parent cell
A group of genetically-identical organisms design in a laboratory using biotechnology.
An organism derived from a single original parent cell
A group of genetically organisms derived from a group of original cell.
- 4. Multiple Choice
Gel electrophoresis is a laboratory technique used to
Replicate DNA under laboratory conditions
Separate DNA fragments based on their size
Replicate DNA or RNA under laboratory conditions
Separate and isolate proteins or DNA fragments based on size
- 5. Multiple Choice
The transfer of genes between species is called gene modification, and the new organism created is called a
Plasmid
Vector
Transgenic
Restriction enzyme
- 6. Multiple Choice
Certain animal species can also reproduce asexually, via a variety of different mechanisms. Even human beings are capable of creating genetic clones.
True
False
- 7. Multiple Choice
This image is a photograph of DNA fragments that have been separated. Identify which strand of DNA is the shortest.
A
B
C
D
- 8. Multiple Choice
A scientist has recovered a piece of tissue from the 100-year-old preserved skin of an extinct Tasmanian wolf (thylacine). To compare a specific region of the sample DNA with DNA from living marsupials, which of the following would be most useful for increasing the amount of wolf DNA available for testing?
DNA profiling
Karyotyping
PCR
Gel electrophoresis
- 9. Multiple Choice
Which is NOT a standard part of PCR?
Use of a heat-resistant polymerase
Breaking of the hydrogen bonds that connect two complementary DNA strands
upply of large numbers of A, C, G and T nucleoside triphosphates
Breaking of covalent bonds within the backbone of DNA strands
- 10. Multiple Choice
Why are plasmids useful in genetic modification of bacteria?
They are the only DNA sequences vulnerable to restriction endonucleases.
They are commonly transferred between different species from all domains of life.
They contain all the essential genes of the bacterium so the target gene becomes essential.
They are easily transferred between bacterial cells and are small enough to manipulate easily.
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