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- 1. Multiple ChoiceA restriction enzyme is used to cut both the gene of interest and the plasmid at the restriction sites.TrueFalse
- 2. Multiple ChoiceIn bacteria, restriction enzymes are used for defense. They cut up the DNA of a virus that infects bacteria.TrueFalse
- 3. Multiple ChoiceOnce the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.polymeraseligasedehydrogenaselactase
- 4. Multiple ChoiceWhich of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?rDNA technologytransferrencetransformationrDNA plasmid
- 5. Multiple Choice
The cell that able to receive recombinant DNA for cloning purpose known as
vector
target DNA
plasmid
host cell
- 6. Multiple Choice
Which of the following is NOT a tool used in genetic engineering?
plasmid
restriction enzyme
DNA helicase
DNA ligase
- 7. Multiple Choice
What type of cut is this photo?
Blunt
sticky
- 8. Multiple Choice
From where does Taq polymerase come?
Bacteria that live in hydrothermal vents/hot springs.
Cambodia
The rain forest
- 9. Multiple Choice
At what temperature does the denaturation step of PCR occur?
95° C
50° C
72° C
- 10. Multiple ChoiceWhich of the following best describes the role of primers?Frame the beginning and end of the target sequenceCopy the DNACause DNA strands to separateHeat the sample
- Answer choicesTagsAnswer choicesTags