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- 1. Multiple Choice
Which of the following describes how a plasmid and gene of interest are combined to create a vector?
A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkage
A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid
A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages
A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid
- 2. Multiple Choice
Which of the following describes why Taq polymerase is required for PCR?
The Taq polymerase is able to unwind the DNA double helix reducing the need of additional enzymes
The Taq polymerase binds to the DNA without a required primer facilitating the process of PCR.
The process of PCR requires prokaryotic polymerase as it is less complex.
The process of PCR requires extreme high temperatures that would denature other polymerases.
- 3. Multiple Choice
Which of the following describes the orientation of the DNA found in a run gel electrophoresis?
Small fragments are found near the well since it is negatively charged
Large fragments are found near the well while small fragments are found at the other end.
Small fragments are found near the well while small fragments are found at the other end.
Large fragments are found near the well since it is positively charged
- 4. Multiple Choice
Which of the following describes why some bands are darker in a gel electrophoresis?
This is a location where there is a secondary well in the gel
There are a large amount of fragments the same size
The DNA at that band absorbed more dye since it is more positively charged
This is an error in the gel electrophoresis caused by a clogged pore in the gel
- 5. Multiple Choice
If one fragment was placed in the thermocycler for a PCR experiment, how many fragments would exist after 5 cycles?
10
16
64
32
- 6. Multiple Choice
Which of the following describes why primers must be added to the thermocycler during a PCR?
Polymerase requires an open 3' end
Polymerase requires RNA for signaling
Polymerase requires an RNA template
Polymerase requires an open 5' end
- 7. Multiple Choice
An experimenter performed a transformation experiment. Which of the following describes the function of the
-plasmid/LB and +plasmid/LB plates?
To compare between the bacteria with and without the plasmid
To determine whether LB is required for the bacterial growth
To ensure the bacteria is viable after experimental set-up
To test whether temperature has an effect on growth
- 8. Multiple Choice
Which of the following describes the minimum number of restriction sites on a plasmid if there are 3 fragments on the gel electrophoresis?
1
2
3
4
- 9. Multiple Choice
Which of the following is responsible for determining the order of nucleotides in a DNA molecule?
DNA sequencing
Gel electrophoresis
Polymerase Chain Reaction
Bacterial Transformation
- 10. Multiple Choice
Which of the following allow for amplification of a DNA fragment abiotically?
DNA sequencing
Gel electrophoresis
Polymerase Chain Reaction
Bacterial Transformation
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