Biology

9th -

12thgrade

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Quick 10Q: BioTechnology

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  • 1. Multiple Choice
    30 seconds
    1 pt

    Which of the following describes how a plasmid and gene of interest are combined to create a vector?

    A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkage

    A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid

    A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages

    A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid

  • 2. Multiple Choice
    30 seconds
    1 pt

    Which of the following describes why Taq polymerase is required for PCR?

    The Taq polymerase is able to unwind the DNA double helix reducing the need of additional enzymes

    The Taq polymerase binds to the DNA without a required primer facilitating the process of PCR.

    The process of PCR requires prokaryotic polymerase as it is less complex.

    The process of PCR requires extreme high temperatures that would denature other polymerases.

  • 3. Multiple Choice
    30 seconds
    1 pt

    Which of the following describes the orientation of the DNA found in a run gel electrophoresis?

    Small fragments are found near the well since it is negatively charged

    Large fragments are found near the well while small fragments are found at the other end.

    Small fragments are found near the well while small fragments are found at the other end.

    Large fragments are found near the well since it is positively charged

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