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Method in Gene Cloning
2 hours ago by
arryana
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  • Question 1
    45 seconds
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    Q.

    First stage involved in gene cloning.

    answer choices

    Amplification of the target gene

    Insertion of the target gene

    Polymerase Chain Reaction

    Isolation of the target gene

  • Question 2
    45 seconds
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    Q.

    The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.

    answer choices

    Cloning

    insertion

    Transformation

    Isolation

  • Question 3
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    Q.

    Types of plasmids produced after insertion of the target gene into a vector.

    answer choices

    Recombinant plasmids

    Non-recombinant plasmids

    Bacterial plasmids

    Isolated plasmids

  • Question 4
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    Q.

    Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?

    answer choices

    using recombinant DNA to produce human hormones from bacteria

    testing parents for genetic disorders before they have children

    engineering fruits and vegetables that resist insects and other pests

    C. developing ways to identify criminals through DNA fingerprinting

  • Question 5
    45 seconds
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    Q.

    How do scientists cut DNA into smaller strands?

    answer choices

    restriction enzymes

    scissors

    ligase

    agarose

  • Question 6
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    Q.

    In order to insert a human gene into plasmid, both must

    answer choices

    code for the same gene product.

    be cut by the same restriction enzyme.

    originate from the same type of cell.

    have identical sequences.

  • Question 7
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    Q.

    Which enzyme "glues" DNA fragments together during insertion?

    answer choices

    restriction enzymes

    DNA ligase

    DNA polymerase

    DNA helicase

  • Question 8
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    Q. Where is DNA located in a bacterial cell?
    answer choices
    nucleus
    ribosomes
    cell membrane
    cytoplasm
  • Question 9
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    Q. _________ DNA is created by using the ______ from a bacteria and splicing a section of DNA into it.
    answer choices
    recombinant, plasmid
    Cloning, ribosome
    transcription, translation
    cumulus, mitochondria
  • Question 10
    45 seconds
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    Q.

    Which of the following could be used as a vector for a human gene?

    answer choices

    Bacterial Plasmid

    Bacteria

    A mitochondria

    mRNA

  • Question 11
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    Q.

    The site where restriction enzymes cut are a called:

    answer choices

    cut sites

    restriction sites

    recognition sites

    cleave sites

  • Question 12
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    Q.

    Sticky ends are produced when the restriction enzyme cuts in a:

    answer choices

    staggered manner

    straight through the DNA

  • Question 13
    45 seconds
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    Q.

    How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?

    answer choices

    1 - through the gene

    2 - on either side of the gene

    3 - through the gene and on either side of the gene

    4 - twice on each side of the gene

  • Question 14
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    Q.

    Cell that receive recombinant DNA for cloning purpose

    answer choices

    Host cell

    plasmid

    DNA target

    Gene of interest

  • Question 15
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    Q.

    Which of the following is NOT a tool used in recombinant DNA technology?

    answer choices

    Cloning vector

    RNA polymerase

    Target DNA

    DNA ligase

  • Question 16
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    Q.

    Which of the following palindromic base sequence identify by EcoRI

    answer choices

    5' GATTTC 3'

    5' GATTCC 3'

    5' GAATCC 3'

    5' GAATTC 3'

  • Question 17
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    Q.

    What is recombinant DNA

    answer choices

    DNA from 2 different organisms combined

    DNA from 2 different ribosomes

    DNA that is separated out from a vector

  • Question 18
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    Q.

    The following are the steps taken during the cloning process.

    1. Transformation 2. Insertion 3. Isolation 4. Screening 5. Cut

    answer choices

    3, 2, 5, 1, 4

    3, 2, 5, 4, 1

    3, 5, 2, 1, 4

    3, 5, 2, 4, 1

  • Question 19
    45 seconds
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    Q.

    Recombinant DNA technology involves a screening process. What is the purpose of screening process?

    answer choices

    Isolate recombinant DNA from bacteria

    Identify cell with target DNA to be cut by restriction enzyme

    Identify bacteria with plasmid to be used as vector

    Identify bacteria with recombinant plasmid

  • Question 20
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    Q.

    The restriction site recognised by SmaI is

    answer choices

    GGATCC

    GGGCCC

    CCCGGG

    GAATTC

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