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- 1. Multiple Choice
First stage involved in gene cloning.
Amplification of the target gene
Insertion of the target gene
Polymerase Chain Reaction
Isolation of the target gene
- 2. Multiple Choice
The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.
Cloning
insertion
Transformation
Isolation
- 3. Multiple Choice
Types of plasmids produced after insertion of the target gene into a vector.
Recombinant plasmids
Non-recombinant plasmids
Bacterial plasmids
Isolated plasmids
- 4. Multiple Choice
Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?
using recombinant DNA to produce human hormones from bacteria
testing parents for genetic disorders before they have children
engineering fruits and vegetables that resist insects and other pests
C. developing ways to identify criminals through DNA fingerprinting
- 5. Multiple Choice
How do scientists cut DNA into smaller strands?
restriction enzymes
scissors
ligase
agarose
- 6. Multiple Choice
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
- 7. Multiple Choice
Which enzyme "glues" DNA fragments together during insertion?
restriction enzymes
DNA ligase
DNA polymerase
DNA helicase
- 8. Multiple ChoiceWhere is DNA located in a bacterial cell?nucleusribosomescell membranecytoplasm
- 9. Multiple Choice_________ DNA is created by using the ______ from a bacteria and splicing a section of DNA into it.recombinant, plasmidCloning, ribosometranscription, translationcumulus, mitochondria
- 10. Multiple Choice
Which of the following could be used as a vector for a human gene?
Bacterial Plasmid
Bacteria
A mitochondria
mRNA
- 11. Multiple Choice
The site where restriction enzymes cut are a called:
cut sites
restriction sites
recognition sites
cleave sites
- 12. Multiple Choice
Sticky ends are produced when the restriction enzyme cuts in a:
staggered manner
straight through the DNA
- 13. Multiple Choice
How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?
1 - through the gene
2 - on either side of the gene
3 - through the gene and on either side of the gene
4 - twice on each side of the gene
- 14. Multiple Choice
Cell that receive recombinant DNA for cloning purpose
Host cell
plasmid
DNA target
Gene of interest
- 15. Multiple Choice
Which of the following is NOT a tool used in recombinant DNA technology?
Cloning vector
RNA polymerase
Target DNA
DNA ligase
- 16. Multiple Choice
Which of the following palindromic base sequence identify by EcoRI
5' GATTTC 3'
5' GATTCC 3'
5' GAATCC 3'
5' GAATTC 3'
- 17. Multiple Choice
What is recombinant DNA
DNA from 2 different organisms combined
DNA from 2 different ribosomes
DNA that is separated out from a vector
- 18. Multiple Choice
The following are the steps taken during the cloning process.
1. Transformation 2. Insertion 3. Isolation 4. Screening 5. Cut
3, 2, 5, 1, 4
3, 2, 5, 4, 1
3, 5, 2, 1, 4
3, 5, 2, 4, 1
- 19. Multiple Choice
Recombinant DNA technology involves a screening process. What is the purpose of screening process?
Isolate recombinant DNA from bacteria
Identify cell with target DNA to be cut by restriction enzyme
Identify bacteria with plasmid to be used as vector
Identify bacteria with recombinant plasmid
- 20. Multiple Choice
The restriction site recognised by SmaI is
GGATCC
GGGCCC
CCCGGG
GAATTC
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