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- 1. Multiple Choice
Semi-conservative DNA replication was proved by the experiments of:
Hershey & Chase
Meselson & Stahl
Watson & Crick
Franklin & Gosling
- 2. Multiple Choice
The Meselson-Stahl experiment demonstrated that DNA replication produces two DNA molecules each composed of
Two old strands
Two new strands
One old strand and one new strand
two strands with variable proportions of new and old DNA.
- 3. Multiple Choice
Starting with 15N15N (heavy) DNA, after ONE round of replication in the 14N medium, the second generation Escherichia coli cells will contain
25% 15N15N DNA, 50% 15N14N DNA, and 25% 14N14N DNA.
50% 15N15N DNA and 50% 14N14N DNA.
100% 15N14N DNA.
50% 15N14N DNA and 50% 14N14N DNA.
- 4. Multiple Choice
Starting with 15N15N (heavy) DNA, and after two replications in the 14N medium, the THIRD generation E. coli cells will contain
25% 15N15N DNA, 50% 15N14N DNA, and 25% 14N14N DNA.
50% 15N15N DNA and 50% 14N14N DNA.
100% 15N14N DNA.
50% 15N14N DNA and 50% 14N14N DNA.
- 5. Multiple Choice
Starting with 15N15N (heavy) DNA, and after two replications in the 14N medium, the THIRD generation E. coli cells will produce the following band(s) in density-gradient centrifugation:
a single band of DNA with medium density.
one band of light DNA and one band of DNA with medium density.
one band of heavy DNA and one band of DNA with medium density.
one band of heavy DNA and one band of light DNA.
- 6. Multiple Choice
Which model does NOT predict that after one replication both DNA molecules will be a 50/50 mix of original and new DNA?
conservative
semi-conservative
dispersive
- 7. Multiple Choice
In the Meselson-Stahl experiment, E.coli was transfered from 15N medium to 14N medium. Centrifugation of DNA after 20 minutes in 14N produced ____ and DISproved the ____ model.
1 intermediate band, conservative
1 intermediate band, dispersive
1 heavy band and 1 light band, semi-conservative
1 light and 1 intermediate band, dispersive
- 8. Multiple Choice
In the Meselson-Stahl experiment, E.coli was transferred into 14N medium from 15N medium. Centrifugation of DNA after 40 minutes in 14N produced ____ and proved the ____ model.
1 light and 1 intermediate band, semiconservative
1 light and 1 intermediate band, conservative
1 intermediate band, dispersive
1 heavy and 1 light band, semi-conservative
- 9. Multiple Choice
What is the end result of one round of DNA replication?
2 semi-conservative strands of DNA
1 semi-conservative strand of DNA
Half of a semi-conservative strand.
Nothing is created
- 10. Multiple Choice
This is the correct model for DNA Replication.
True
False
- 11. Multiple Choice
This is the correct model for DNA Replication.
True
False
- 12. Multiple Choice
This is the correct model for DNA Replication.
True
False
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