Have an account?
Skip to Content
Build your own quiz
Suggestions for you
42 questions
Show Answers
See Preview
- 1. Multiple Choice
Which of the following best describes a DNA molecule?
antiparallel double helix
contains ribose
made of amino acids
contains Uracil
- 2. Multiple ChoiceWhich sequence of DNA bases would pair with this partial strand
ATG TGA CAGATG TGA CAGTAC ACT GTCGTA AGT GACCAT TCA CTG - 3. Multiple ChoiceSeals the Okazaki fragments togetherDNA Polymeraseligasehelicaseprimase
- 4. Multiple Choice
This repetitive sequences of DNA on the ends of chromosomes (TTAGGG) for protection.
telomeres
telomerase
Okazaki fragments
primer
- 5. Multiple ChoiceWhich enzyme is needed to start DNA replication?DNA PolymeraseTopoisomeraseLigasePrimase
- 6. Multiple ChoiceWhat is meant by the description "antiparallel" regarding the strands that make up DNA?The twisting nature of DNA creates nonparallel strands.One strand is positively charged and the other is negatively charged.The 5' to 3' direction of one strand runs counter to the 5' to 3' direction of the other strand.Base pairings create unequal spacing between the two DNA strands
- 7. Multiple ChoiceWhat is meant that DNA replication follows a “semi-conservative” model?After DNA replication, each strand contains one side that is a parental strand and the other side is a daughter strand.After DNA replication, one completed strand contains only parental DNA while the other contains only daughter DNA.None of these identify the “semi-conservative” model.After DNA replication, one completed strand contains a mixture of both parental DNA and daughter DNA dispersed throughout the strand.
- 8. Multiple ChoiceWhere does transcription occur in eukaryotic cells?NucleusRibosomeCytoplasmRough E.R.
- 9. Multiple ChoiceWhat is the complementary tRNA anticodon to the following mRNA codon: AUGUACTACATGAAC
- 10. Multiple Choice
Transcribe the following DNA sequence:
TAT GGC ACT
TAT GGC ACT
ATA CCG TGA
AUA CCG UGA
UAU GGC ACU
- 11. Multiple Choice
Translate the following RNA sequence:
AUG AAU UGU UGA
Met-Phe-Cys-STOP
Met-Asn-Cys-STOP
Met-Asn-Cys-Trp
Tyr-Leu-Thr-Thr
- 12. Multiple ChoiceA frameshift mutation could result froma base insertion only.a base deletion only.a base substitution only.either an insertion or a deletion of a base.
- 13. Multiple ChoiceThe anticodon of a particular tRNA molecule iscomplementary to the corresponding mRNA codon.complementary to the corresponding triplet in rRNA.the part of tRNA that bonds with a specific amino acid.catalytic, making the tRNA a ribozyme.
- 14. Multiple ChoiceIn eukaryotic cells, transcription cannot begin untilthe two DNA strands have completely separated and exposed the promoter.several transcription factors have bound to the promoter.the 5' caps are removed from the mRNA.the DNA introns are removed from the template.
- 15. Multiple ChoiceOriginal: ATC CAT
Mutation: ATC GCAT
What mutation occurred?deletioninsertionsilenttransverse - 16. Multiple ChoiceIn RNA, Uracil pairs with ______.cytosineadenineguaninethymine
- 17. Multiple ChoiceThe process to go from DNA to mRNA is _______translationtranscriptionreplication
- 18. Multiple ChoiceThe process to go from mRNA to a protein is called ____transcriptionrRNAreplicationtranslation
- 19. Multiple ChoiceWhich component of DNA determines the traits of an organism?Nitrogen BasesSugar-Phosphate BackboneHydrogen bonds
- 20. Multiple ChoiceWhat is the location in the cell for translation?nucleusmitochondriaribosomechloroplast
- 21. Multiple ChoiceWhat is the three base sequence of mRNA that codes for a single amino acid?AnticodonCodonProtein
- 22. Multiple Choice
Which of the following does not occur in prokaryotic gene expression but does occur in eukaryotic gene expression?
mRNA, tRNA, and rRNA are transcribed
RNA polymerase binds to the promotor
a poly-A tail is added to the ends of the mRNA
Translation can begin as soon as transcription has begun even a little
- 23. Multiple ChoiceWhich of the follwoing best describes the significance of the TATA box in eukaryotic promoters?it is the recognition site for a specific transcription factorit sets the reading frame of the mRNAit is the recognition site for ribosomal bindingit signals the end of the nucleotide sequence of the gene
- 24. Multiple ChoiceWhat is the function of the poly-A tail in mRNA?to add modified guanin to the 3' end of the mRNAto indicate the site of translational terminationto code for the binding of RNA polymerase to the DNAto help protect the mRNA from degradation by hydrolytic enzymes
- 25. Multiple ChoiceWhat is a ribozyme?enzyme that uses RNA as a substrateRNA with enzymatic activityan enzyme that catlyzes the association between the large and small ribosomal subunitsan enzyme tha synthesizes RNA primers during DNA replication
- 26. Multiple ChoiceWhat must happen to a newly made polypeptide before it can be secreted from a cell?it must be translated by a ribosome that remains free of attachment to the ERits signal sequence must target it to the ER, after which it goes to the GolgiIts signal sequence must be cleaved offIts signal sequence must target it to the plasma membrane
- 27. Multiple ChoiceWhat is the effect of a nucleotide-pair substitution that results in a nonsense mutation in a gene?it changes an amino acid in the encoded proteinIt has no effect on the amino acid sequence of the encoded proteinit introduces premature stop codon into the mRNAit alters the reading frame of the mRNA
- 28. Multiple ChoiceWhich is the best description of a gene?a DNA sequence that is expressed to form a functional product; either RNA or polypeptidea DNA subunit that codes for a single complete proteina DNA-RNA sequence combination that results in an enzymatic producta descrete unit of hereditary information that consists of a sequence of amino acids
- 29. Multiple ChoiceA possible sequence of nucleotides in the template strand of DNA that would code for the polypeptide sequence phe-leu-ile-val would be?5'-TTG-CTG-CAG-TAG-3'3'-AAC-GAC-GUC-AUA-5'3'-AAA-AAT-ATA-ACA-5'3'-AAA-GAA-TAA-CAA-5'
- 30. Multiple ChoiceWhat amino acid sequence will be produced based on the mRNA codon sequence
5'-AUG-UCU-UCG-UUA-UCC-UUG-3'met-ser-leu-ser-leu-sermet-ser-ser-leu-ser-leumet-leu-phe-arg-glu-glumet-glu-arg-arg-glu-leu - 31. Multiple Choice
Students subjected three samples of five different molecules to gel electrophoresis as shown in the Figure.
Which of the following statements best explains the pattern seen on the gel with regard to the size and charge of molecules A and B?
Molecules A and B are positively charged, and molecule A is smaller than molecule B.
Molecules A and B are positively charged, and molecule A is larger than molecule B.
Molecules A and B are negatively charged, and molecule A is smaller than molecule B.
Molecules A and B are negatively charged, and molecule A is larger than molecule B.
- 32. Multiple Choice
Which of the technologies listed below is a valuable method for mass-producing genes and other useful proteins?
gene targeting
recombinant DNA technology
gel electrophoresis
metabolism
- 33. Multiple Choice
What is a restriction enzyme?
An enzyme that cuts DNA
An enzyme that add to the DNA strand
An enzyme that builds proteins
An enzyme that breaks down lipids
- 34. Multiple ChoiceBased on these DNA fingerprints, whose blood was found in the blood stain at the crime scene?BobSueJohnLisa
- 35. Multiple Choice
In gel electrophoresis, the largest DNA fragment will appear
closest to the starting wells
farthest from the starting wells
three quarters away from the starting wells
it depends on how many fragments there are
- 36. Multiple ChoiceWhat is the end goal of PCR?Look at banding patterns of DNAIncrease amount of DNADecrease the amount of DNAFingerprinting
- 37. Multiple Choice
Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location?
bacteriophage
restriction enzyme
vector
plasmid
- 38. Multiple Choice
In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized above. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria.
Plates that have only ampicillin-resistant bacteria
growing include which of the following?
I only
III only
IV only
I and II
- 39. Multiple Choice
In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized above. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria.
Which of the following best explains why there is no growth on plate II?
The initial E. coli culture was not ampicillin resistant.
The transformation procedure killed the bacteria.
Nutrient agar inhibits E. coli grow
The bacteria on the plate were transformed.
- 40. Multiple Choice
In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized above. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria.
Plates I and III were included in the experimental
design in order to
demonstrate that the E. coli cultures were viable.
demonstrate that the plasmid can lose its ampr gene.
demonstrate that the plasmid is needed for E. coli growth.
prepare the E. coli for transformation.
- 41. Multiple Choice
In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized above. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria.
Which of the following statements best explains why there are fewer colonies on plate IV than on plate III?
Plate IV is the positive control.
Not all E. coli cells are successfully transformed.
The bacteria on plate III did not mutate.
The plasmid inhibits E. coli growth.
- 42. Multiple Choice
In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized above. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria.
In a second experiment, the plasmid contained the gene for human insulin as well as the ampr gene. Which of the following plates would have the highest percentage of bacteria that are expected to produce insulin?
I only
III only
IV only
I and III
- Answer choicesTagsAnswer choicesTags