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44 questions
Group of structural and regulating genes that functions as a single unit
Operon
Plasmid
DNA
Oncogene
In an operon, a sequence of DNA where RNA polymerase begins transcription
Repressor
Inducer
Structurel Gene
Promoter
In an analysis of the nucleotide composition of DNA, which of the following will be found
A=C
A+G = C + T
G + C = A + T
A = G and C = T
An amino acid deficiency in an organism is most likely to affect
post translation
translation
post transcription
transcription
Amino acid tryptophan is added to the agar the E.coli are growing on, the trp operon will be
not affected
increased in function
repressed
induced
DNA wrapped around histone proteins
nucleosome
Barr body
helicase
primase
The pink ribbon-like structure is
tRNA
DNA
mRNA
rRNA
Small, circular DNA molecules from bacteria are called...
Chromosomes
Chromatin
Transcripts
Plasmids
In the diagram, what is structure #1?
Topisomerase
Primase
Helicase
ssBP
In the diagram what is #4?
Topisomerase
Nucleotide
Helicase
Primase
In DNA replication, what enzyme unzips the double helix?
Primase
Topisomerase
DNA Polymerase I
Helicase
In DNA replication, which occurs first?
DNA polymerase adds new nucleotides
DNA polymerase replaces RNA with DNA
DNA ligase binds segments together
Primase creates a primer
What is the orientation of DNA strands?
Single strand, 5'-3'
Double strand, both 5'-3'
Double strand, antiparallel
Double strand, semiconservative
Which strand of these DNA molecule would be the lagging strand?
Top
Bottom
If a polypeptide chain has 100 amino acids, then how many nucleotides does the mRNA contain?
99
100
299
300
What is the correct order of function in replication?
helicase, ssbp, topoisomerase, DNA primer
helicase, topoisomerase, ssbp, RNA primer
ssbp, helicase, topoisomerase, DNA primer
helicase, ssbp, topoisomerase, RNA primer
A ribosome has three binding sites for tRNA. Match the site with the correct function.
P site holds tRNA that carries next amino acid added to chain
A site is where discharged tRNAs leave the ribosome
A site holds tRNA that carries next amino acid added to chain
E site holds tRNA that carries the growing amino acid chain
Base substitutions of the 3rd base of codon rarely result in an error in polypeptide because...
substitutions are corrected before transcription begins
substitutions are restricted to introns
transcribed errors attract snRNP’s,stimulating splicing
base pair rule less strict for the 3rd base codon/anticodons
The anticodon of a particular tRNA molecule is...
complementary to the corresponding mRNA codon
complementary to the rRNA triplet
part of tRNA that bonds to a specific amino acid
changeable, depends on the amino acid that bonds to the tRNA
Using the picture what polypeptide does CAA-CGG-GUA code for?
His, Arg, Stop
Gln, Arg, Val
Gln, His, Met
Met, Gln, Arg
Which of the following mutations would be most likely to have a harmful effect on an organism?
a base pair substitution
a deletion of three nucleotides near the middle of a gene
a nucleotide deletion at the end of the gene
single nucleotide inserted close to start of coding sequence
Identify which of the following best describes purines and pyrimidines
Purines (C, T, & U) have single ring, Pyrimidines (A & G) have double ring
Purines (A & G) have single ring, Pyrimidines (C, T, & U) have double ring
Purines (C, T, & U) have double ring, Pyrimidines (A & G) have single ring
Purines (A & G) have double ring, Pyrimidines (C, T, & U) have single ring
Which of the following demonstrates base pairing rules to 5' - CAGGT - 3'
3' - TGGAC - 5
5' - GTCCA - 3'
3' - GTCCA - 5'
5' - CAGGT - 3'
Describe the difference between the synthesis of the leading and lagging strands.
directionality: leading = 3' -> 5', lagging = 5' -> 3'
directionality: leading = 5' -> 3', lagging = 3' -> 5'
synthesis continuity: leading = discontinuous & lagging = continuous
synthesis continuity: leading = continuous & lagging = discontinuous
How do the three types of RNA work together?
mRNA carries message, tRNA carries amino acids, and rRNA makes ribosome
mRNA carries amino acids, tRNA transfer message, and rRNA makes ribosome
mRNA makes ribosomes, tRNA carries amino acids, and rRNA relays information
mRNA is made, tRNA holds the DNA in place, and rRNA removes the deoxyribose
Which of the following are NOT post-transcriptional modifications?
5' cap
TATA box
poly-A tail
intron removal
Which of the following describes epigenetic changes that affect gene expression?
alternative RNA splicing
post-transcriptional modification
DNA methylation or histone acetylation
operon inhibition
Which of the following genetic engineering techniques is incorrectly described?
Electrophoresis separates molecules based on size & charge
PCR allows for DNA fragments to be stabilized for engineering processes
Bacterial transformation introduces DNA to bacterial cells
DNA sequencing determines the order of nucleotides in a DNA molecule
What is transcribed from DNA to produce a protein and is a good indication of whether or not a particular gene is expressed?
mRNA
cDNA
tRNA
trRNA
From microarray data, what can we conclude?
genes that cause cancer
which genes may be affected in cancer
genes that stop cancer
which cells have genes
This is the practice of inserting the DNA of another species into an organism's DNA so that the organism gains a new trait.
Cloning
Gel Electrophoresis
Genetic Engineering
DNA Fingerprinting
Which kitten is most likely the offspring of this cat?
1
2
3
4
What is the process in which an unspecialized stem cell turns into a specialized body cell?
DNA Replication
Differentiation
Respiration
Genetic Modification
During gel electrophoresis, the _______ pieces of DNA move farthest down the gel
lightest
heaviest
ugliest
deadliest
What does transgenic mean?
An organism that can change its genes at will
A stem cell that can differentiate into anything
An organism that has had another species' DNA put in it
A bacteria that reproduces asexually
Another name for a point mutation is:
Substitution
Frameshift
Insertion
Deletion
ATTTGAGCC- Original
ATTGAGCC - Mutated
The example above is an example of a
Insertion
Point mutation
Deletion
Substitution
What type of Mutation is this to the sentence "THE CAT WAS FUN":
THE BAT WAS FUN
Substitution Mutation
Deletion Mutation
Insertion Mutation
What type of Mutation is this to the sentence "THE CAT WAS FUN":
THE CAT AWA SFU N...
Substitution Mutation
Deletion Mutation
Insertion Mutation
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