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34 questions
What suffix is commonly applied to enzymes?
-ase
-ose
-ter
-ice
Enzymes act as catalysts to __________ the rate of a biochemical reaction by ___________ the activation energy needed for the reaction to occur.
increase, lowering
decrease, lowering
increase, raising
decrease, raising
The induced fit model is preferred over the lock-and-key model because
some enzymes catalyze multiple reactions.
all substrates have the same shape.
the active site is a polar region.
the enzyme and substrate match each other structurally and chemically.
According to the induced fit model, as a substrate approaches an enzyme, it induces a ______________ in the active site.
conformational change
chemical change
color change
After an enzyme is denatured, the substrate can ______ to the active site.
bind
no longer bind
The loss of enzyme shape is called
denaturation.
stress.
random movement.
alignment.
What compound is found in the cells of many fruits and vegetables. When this chemical comes into contact with catecholase and oxygen, it reacts to form benzoquinone and water?
lactose
glucose
catechol
galactose
catechol + 2 O2 + ______________ → Benzoquinone + water
catecholase
water
hydrogen peroxide
sulfuric acid
catechol + 2O2 + catecholase → ___________________ + water
Benzoquinone
Catechol
hydrogen peroxide
sulfuric acid
catechol + 2O2 + catecholase → Benzoquinone + ___________
Benzoquinone
Catechol
water
sulfuric acid
When the product benzoquinone gets exposed to air, it converts to a pigment that is responsible for the dark coloration of bruised fruits and vegetables. What is the name of that pigment?
melanin
carotene
chlorophyll
anthocyanin
Of the steps listed, which would you do first?
Allow the Spec to warm up per manufacturer's instructions
Wipe the blank cuvette to remove fingerprints
With the cuvette removed, set the absorbance to 0
Insert the blank cuvette and calibrate the spec.
A spectrophotometer can be used to (check all that apply):
determine the presence of a substance in a solution.
measure the concentration of an unknown solution.
determine the molecular composition of an unknown substance.
identify an unknown substance
The amount of light absorbed by a sample is called its:
Absorbance
Transmittance
Hue
Saturation
A small container that is used to hold samples intended for spectroscopic analysis:
Cuvette
Test Tube
Microcentrifuge Tube
PCR Tube
The amount of light that passes through a sample is called:
Transmittance
Absorbance
Radiation
Quantum force
Which answer has the steps for using the spectrophotometer in the correct order?
Set wavelength → place sample in machine → close lid → zero out the machine → place blank in machine → close lid → take reading
Set wavelength → place blank in machine → close lid → zero out the machine → place sample in machine → close lid → take reading
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