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10 questions
Southern blot is the technique for _____________ blot
RNA
Lipid
DNA
Protein
_________ enzyme is used in the elongation/ extension of DNA fragment in PCR reaction
DNA/ Tag Polymerase
Revers transcriptase
E. Co RI
All
_________ media is used for hybridoma cell culture.
HAT
CHO-S
Feed - 220
Leibovitz L-15
In Southern blotting _________ type of gel is used for the separation of molecular band
Polyacrylamide
Agarose
Starch
None
The technique PCR was discovered by the scientist __________ in 1985
Georges & Cesar
Alac Jeffry
E. M. Southern
Kary Mullis
In PCR denaturation of DNA is take place in ________ 0C temperature
72 0C
940C
40 to 550C
1200C
The enzyme DNA polymerase used in PCR reaction is isolated from which one of the
following bacteria
Thermus aquaticus
Plasmodium vivax
Both
a) None
In PCR reaction, how much kinds of nucleotide are used
5
4
8
2
In Southern blotting, ___________ type paper is used for blot transfer
Nitrocellulose membrane or Nylon membrane filter
Whatman filter paper
Paper towel filter paper
None
_________ in 1975 developed the procedure for DNA transfer or for the DNA blot
Kary Mullis
E. M. Southern
Alac Jeffry
Georges & Cesar
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