Bacterial Transformation
44 minutes ago
rhusselstein
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12 QuestionsShow answers
  • Question 1
    120 seconds
    Q.

    What is transformation?

    answer choices

    The use of viruses to transform or genetically engineer cells.

    The measure of how well cells are transformed in a new phenotype.

    The insertion of a plasmid with a foreign gene into a bacterial cell which results in new acquired traits.

    Genetic engineering or transformation of mammalian cells.

  • Question 2
    30 seconds
    Q.

    When fragments of DNA are connected from different sources, this creates:

    answer choices

    A plasmid

    A clone

    Recombinant DNA

    Fragmented DNA

  • Question 3
    30 seconds
    Q.

    Bacterial DNA that is often circular or in the shape of a ring is called a:

    answer choices

    Clone

    Plasmid

    Fragment

    Recombinant DNA

  • Question 4
    120 seconds
    Q.

    Crops are often genetically engineered for improved traits. Such crops and organisms are called:

    answer choices

    Genetically modified organisms

    Clones

    Transformed

    Nutritionally improved

  • Question 5
    30 seconds
    Q.

    DNA can be cut with

    answer choices

    ligase

    helicase

    restriction enzymes

    DNA scissors

  • Question 6
    120 seconds
    Q.

    Cells are made competent (ready to be transformed) by

    answer choices

    The incubation of the bacteria at 95C

    A process that uses calcium chloride.

    A process that uses GFP

    A process that uses polymerases

  • Question 7
    120 seconds
    Q.

    Heat shock

    answer choices

    Trap the plasmids inside the cell.

    Creates a temperature gradient, that allows the plasmid to move from into the bacteria through the enlarged pores of the cell membrane.

    Provides for the death of the cell.

    Increases the disintegration of the cell wall.

  • Question 8
    30 seconds
    Q.

    What is the purpose of the recovery period after heat shock during bacterial transformation?

    answer choices

    allows the bacteria to repair its membrane

    allows the bacteria to take up the plasmid DNA

    lets the bacteria get used to the temperature at which it will grow

    lets the bacteria start to produce the product of the antibiotic resistance gene

  • Question 9
    120 seconds
    Q.

    A cell becomes competent when this happens:

    answer choices

    The formed plasmid is inserted into the cell.

    The gene of interest is turned on.

    Super coiled molecules are formed in the plasmid of the cell.

    There's a change in the structure and permeability of the cell membrane.

  • Question 10
    120 seconds
    Q.

    How might a plasmid contribute to a selective advantage for a bacterial cell?

    answer choices

    Make the cell competent.

    Could provide a selective advantage for that cell.

    Make the cell wall stronger.

    Does not contribute

  • Question 11
    30 seconds
    Q.

    Process in which plasmid DNA is taken up by a bacterial or yeast cell.

    answer choices

    transcription

    translation

    transformation

    replication

    reverse transcription

  • Question 12
    30 seconds
    Q.

    Bacteria are model organisms because

    answer choices

    They behave very well in public

    They are manipulated easily and reproduce quickly.

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