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30 questions
Below is the list of laboratory rules except
Wear lab coat, glove and cover shoes every time entering into lab
Never do any experiment without instruction by laboratory instructor/technician
Eating, drinking and smoking are prohibited inside the laboratory
Student can made noise during discussion inside the laboratory
You are asked to handle the concentrated H2SO4 by your instructor. What precaution should you do while handling it?
Pour the acid inside the fume hood, wearing the protective glove and lab coat.
Running the experiment using that acid on the bench.
Pour the acid without wearing glove inside the fume hood
Add the acid with 100% H2O2 inside the fume hood.
During the experiment with bioreactor, you are required to take the sample using the syringe. After finish sampling, what should you do with that syringe?
Wash it and let it dry on the table.
Syringe is consider as single use consumable, it should be dispose inside the biohazard bin after used.
Wash and keep it as souvenir.
Just left it on the table.
What is the proper way to handle acid?
Add acid to water.
Add H2O2 into 2M of HCl
Pour water into acid
Add 2M of H2SO4 into H2O2
When running the experiment, you are planning to continue doing your analysis on the next day. You have taken the sample and want to store it in the chiller. How you should label your sample?
Just label it with "sample A' and stored it
Put your name and date.
Write your name and matric no., sample name, date of collected, estimated expired date, class/course, instructor name.
Just put your group no and date.
All of these are the step you will do in the time of chemical or bacterial sample spill on the table except
Use the spill kits to stop the spill from spreading to other area.
Clean using dry towel and spray it with 70% ethanol for disinfectant.
Let the spill spread and drop into the floor and flooding the area
Alert the lab instructor/technician about the spill.
Which one is the signage for "Extremely Flammable" that you can found in some chemical?
What is the hazard that you may found in the lab.
Chemicals
Infectious bacteria
physical hazard such as falling from the wet floor
All the listed above
What is the classification of Biosafety level 1 laboratory
Involve with no infectious bacteria
The lab activities consider low risk to community
Involve any infectious disease bacteria but will not harm to community.
Involve any infectious disease bacteria and easily been spreading due to contact from one individual to another.
What is Containment Level 1
Need to have special fume hood with special ventilation to avoid spreading the disease to outside environment.
Required special and well-design facilities with HEPA filter and strict laboratory access.
This level of containment represents an isolated unit, functionally and, when necessary, structurally independent of other areas.
Required no special design lab and work can be done on the bench. Example is basic microbiology lab.
What factor that you need to consider when you did the risk assessment involving the experiment inside the laboratory.
Availability of lab equipment.
Space of laboratory
Pathogen and its natural route of infectious.
Presence of your friend.
What you need to do before you start running any experiment in laboratory?
Understand the laboratory rules and safety rules.
Identify the type of equipment need to be used in your experiment.
Read and understand the process involve in each experiment and able to plan your work according to the timeline
Identify the potential hazard through reading the experimental manual.
All the above
Where can you find safety signage in labs
on the wall
Material Safety Data Sheet (MSDS)
OSH and laboratory SOP folder
All the above
What you should do if you find out that the lab equipment is malfunction when you try to run it?
Try to fit it by yourself.
Report to lab instructor/lab technician
Keep use it and ignore the warning.
Tag the equipment clearly so that no one will use it and report the case to lab instructor/lab technician
What is the function of biosafety cabinet?
A primary barrier to reduce the spreading of disease caused by bacteria/microorganism into the laboratory environment.
A work space to culture and sub-culture the bacteria.
A designated space to prevent the cross-contamination due to air borne contaminant during transfer of the bacteria.
Provide extra ventilation to ensure enough sterilization when subculture or transfer the bacteria.
How many ways infectious disease can be spreaded?
1 - airbone
2 - airborne and contact
3 - airborne, contact and oral
4 - airborne, contact, oral and droplet
Which of the following procedures could generate aerosols?
Cell sorters
Pipetting
Sonicating tissue culture cells
All of the above
The acronym HEPA stands for?
High Evaluation Protection
High-Efficiency Particulate Air
High-Energy Particles in Air
Hepatitis A
Which class of biosafety cabinet is the most common and used for working with biological materials or organisms?
Class I
Class II
Class III
Class IV
Biosafety is working with biological material or organisms with potential to cause disease in?
Humans
Animals
Plants
All the above
Stephylococcus Cerevisia would be handled at which Risk Group?
Risk Group 1
Risk Group 2
Risk Group 3
None of the above
For research that requires Biosafety Level 2 containment, Biological Safety Cabinets must be certified by the investigator..........
Daily
Monthly
Annually
Never, it's not important
It is okay to wear Croc sandal with socks to enter laboratory?
True
False
Each time you use centrifuge and sonicate, always de-contaminate it using absolute ethanol.
True
False
Infectious agent, biological materials and consumable items must be disinfected chemically or by autoclave before final disposal in biohazard waste bin.
True
False
All lab personnel working in an area where biological agents are used, must be made aware of any potential hazards associated with them?
True
False
If IIUM Health Centre is closed, you should wait until the next day when they open to seek medical attention?
True
False
Human Cell Lines are treated ad bloodborne pathogens by OSHA
True
False
A Biohazard sign must be completed and posted on lab doors in order to meet Biosafety Level 2 containment requirements.
True
False
When identifying risk and addressing hazards, the goal is to provide the highest __________ and the lowest practical __________.
resistance/virulance
attenuation/pathogenicity
protection/exposure
prevention/virulance
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