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- 1. Multiple Choice
When a positive + result occurs ELISA, then one could conclude that...
a + result always means illness
it is a false + and no illness
the antibody is present
- 2. Multiple Choice
What is a primary antibody?
the second antibody used to detect foreign particle
particle produced by the antigen
the first antibody used to detect foreign particle
second cell produced by the macrophage
- 3. Multiple Choice
Why is a positive and negative control necessary in the setup of ELISA?
positive controls are equal to negative controls
ELISA is subject to errors; if controls fail the results are untrustworthy
ELISA is a well run test that typically does not need controls
negative and positive controls are needed to exclude all results
- 4. Multiple Choice
Why is it necessary to wash the samples repeatedly?
it is important to keep the lab wells clean
it is important to wash away all bound and unbound antibodies from the wells
it is important to wash away the unbound antibodies
washing the wells is just solid lab practice
- 5. Multiple Choice
A serial dilution is
the step wise saturation of a substance in solution
the step wise dilution of a substance in solution
a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.
a dilution that increase by a factor of 1/10 every step
- 6. Multiple Choice
What does the symbol μL stand for?
nanoliters
microliters
muliters
milliters
- 7. Multiple Choice
From what cells do antibodies originate?
b cells
prison cells
t cells
red blood cells
- 8. Multiple Choice
The basic 4 steps of the ELISA are:
bind sample to well
add primary Ab and wash
add secondary Ab/enzyme and wash
add substrate
true
false
- 9. Multiple Choice
what does ELISA stand for
enzyme linked immune sorbent assay
enzyme linked independent sorbent assay
enzyme linked immune system assay
enzyme linked immune stain association
- 10. Multiple Choice
Why do you assay your samples in duplicates?
To act as a control of the assay
To test for pipetting errors
Reproducibility of results
all of the above
- 11. Multiple Choice
Why is there an enzyme attached to the secondary antibody?
They provide a colormetirc indication of antigen exposure
They link to the proteins used
They are used to detect DNA
- 12. Multiple Choice
The first layer on the ELISA plate will be the
antigen
antibody
virus
substrate
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