14 questions
What did CRISPR originally do?
defend bacteria against viruses
create viruses
create bacteria
defend viruses from bacteria
What do CAS1 and CAS2 do?
Identify deadly viruses
Identify an invader's DNA and remove it
Create RNA to kill invaders
Directly kill invaders through endocytosis
What is the protospacer?
a type of DNA that functions in a phage infection
the DNA of the original bacteria
DNA that is mutated through UV rays
Nothing I need to worry about.
What does CAS9 form with the two different types of RNA?
A macrophage
A bacteriophage
a search complex
a virus
What can CAS9 do?
kill an invading bacteriophage
kill viruses by endocytosis
kill the bacteria itself
prevent infection through the first line of defense
What is PAM?
a 2-6 base pair sequence that signals infection
a macrophage
a bacteriphage
a mutated gene sequence that will be destroyed permanently
CRISPR is composed of
the cas9 protein and guide RNA
RNA and ribosomes
bacteria and viruses
cas9, cas 1+2 and ribosomal RNA
What does the cas9 protein do?
cut viruses
Cut RNA
cut DNA
ingest bacteria
What does the guide RNA do?
destroy invaders like bacteriophages immediately
cut invading pieces of RNA
recognize invaders
recognize sequences of DNA to be edited.
What is the first step in using CRISPR?
create an important RNA sequence
find bacteriophages to invade bacteria
identify the sequence causing the health problem
create a virus
What do the spacers contain?
DNA of invading bacteria
DNA of invading viruses
RNA of invading viruses
RNA of invading bacteria
Why is it important to cut the DNA?
to remove the RNA invader
to edit the existing genome
to kill the viruses
to kill the bacteria
In terms of DNA, CRISPR is like
the space bar
cut and paste
delete
document recover
What do scientists hope to do with CRISPR/CAS9
develop advances to mutate our own DNA
develop advances to cure inherited disorders
develop advances to kill bacteria
develop advances to kill all pathogens