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- 1. Multiple Choice
What is your first step?
Label your microcentrifuge tubes with +DNA and -DNA
Transfer 15 colonies of e. coli into your microcentrifuge tubes
Transfer 10 microliters of pGFP
- 2. Multiple Choice
What kind of operon is being used in this experiment?
regulartor
Inducible
Repressible
Transcription
- 3. Multiple Choice
What is IPTG in this picture?
corepressor
inducer
transcription factor
protein
- 4. Multiple Choice
On which of the plates would you expect to have transformed bacterial colonies AND Gene expression of the GFP gene (they'll be glowing)?
-DNA/+Amp
+DNA/+Amp
+DNA/+Amp/+IPTG
- 5. Multiple Choice
What is the purpose of the changes in extreme temperatures?
Kill off the low fitness e. coli
Increase Permability of the membrane
Decrease Permeability of the membrane
allow bineary fission to occur easier
- 6. Multiple Choice
Which tube is pGFP added to?
-DNA
+DNA
Both
- 7. Multiple Choice
What is pGFP?
A plasmid
A chromosome
A protein
- 8. Multiple Choice
Which of the following is not one of the plates we'll be using?
-DNA
+DNA
+DNA/+AMP/+IPTG
+DNA/+AMP
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