Bacterial Transformation
9 months ago
sphar
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10 QuestionsShow answers
  • Question 1
    120 seconds
    Q.

    What is competency for a cell?

    answer choices

    The insertion of a formed plasmid into the cell.

    The chemical process of switching the gene.

    Super coiled molecules formed in the plasmid of the cell.

    Change in the structure and permeability of the cell membrane.

  • Question 2
    120 seconds
    Q.

    How might a plasmid contribute to a selective advantage for a bacterial cell?

    answer choices

    Make the cell competent.

    Could provide a selective advantage for that cell.

    Make the cell wall stronger.

    Does not contribute

  • Question 3
    120 seconds
    Q.

    What is transformation?

    answer choices

    The use of viruses to transform or genetically


    engineer cells.

    The measure of how well cells are transformed in a new phenotype.

    The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

    Genetic engineering or transformation of mammalian cells.

  • Question 4
    120 seconds
    Q.

    Cells are made competent by;

    answer choices

    The incubation of the bacteria at 95C

    A process that uses calcium chloride.

    A process that uses GFP

    A process that uses polymerases

  • Question 5
    120 seconds
    Q.

    Heat shock

    answer choices

    Trap the plasmids inside the cell.

    Enlarges the pores of the cell.

    Provides for the death of the cell.

    Increases the disintegration of the cell wall.

  • Question 6
    120 seconds
    Q.

    The exogenous DNA used in bacterial transformation can be,

    answer choices

    mRNA molecule

    rDNA

    engineered plasmid

    red fluorescent protein

  • Question 7
    120 seconds
    Q.

    LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;

    answer choices

    Grow on the agar plate

    Will not grow on the agar plate.

    will only grow after the plate has been incubated at 45C

    will only grow after the plate has been placed in a warm water bath for 2 minutes.

  • Question 8
    120 seconds
    Q.

    Which of the following statement is true concerning transformation;

    answer choices

    Bacterial transformation involves the transfer of genetic material that results in the acquisition of a new trait that is stable but not inheritable.

    There are no bacterial species that go thru the transformation process naturally.

    Transformation efficiencies are a way to determine how many cells were transformed per microgram of plasmid DNA used.

    The promoter is the region at the end of the gene .

  • Question 9
    120 seconds
    Q.

    Observe the results above. What gene is present in the engineered plasmid?

    answer choices

    kanamycin resistant gene

    ampicillin resistant gene

    tetracycline resistant gene

    No plasmid is necessary to grow on plate 3.

  • Question 10
    180 seconds
    Q.

    Examine the results for group 4. Why would this group see growth without RFP in the third plate?

    answer choices

    The bacteria cells did not transform.

    The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.

    The bacteria cells transformed, but did not incorporate the plasmid with the arabinose gene.

    Group 4 did not observe growth in the third plate.

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