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What is competency for a cell?
The insertion of a formed plasmid into the cell.
The chemical process of switching the gene.
Super coiled molecules formed in the plasmid of the cell.
Change in the structure and permeability of the cell membrane.
How might a plasmid contribute to a selective advantage for a bacterial cell?
Make the cell competent.
Could provide a selective advantage for that cell.
Make the cell wall stronger.
Does not contribute
What is transformation?
The use of viruses to transform or genetically
The measure of how well cells are transformed in a new phenotype.
The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.
Genetic engineering or transformation of mammalian cells.
Cells are made competent by;
The incubation of the bacteria at 95C
A process that uses calcium chloride.
A process that uses GFP
A process that uses polymerases
Trap the plasmids inside the cell.
Enlarges the pores of the cell.
Provides for the death of the cell.
Increases the disintegration of the cell wall.
The exogenous DNA used in bacterial transformation can be,
red fluorescent protein
LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;
Grow on the agar plate
Will not grow on the agar plate.
will only grow after the plate has been incubated at 45C
will only grow after the plate has been placed in a warm water bath for 2 minutes.
Which of the following statement is true concerning transformation;
Bacterial transformation involves the transfer of genetic material that results in the acquisition of a new trait that is stable but not inheritable.
There are no bacterial species that go thru the transformation process naturally.
Transformation efficiencies are a way to determine how many cells were transformed per microgram of plasmid DNA used.
The promoter is the region at the end of the gene .
Observe the results above. What gene is present in the engineered plasmid?
kanamycin resistant gene
ampicillin resistant gene
tetracycline resistant gene
No plasmid is necessary to grow on plate 3.
Examine the results for group 4. Why would this group see growth without RFP in the third plate?
The bacteria cells did not transform.
The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.
The bacteria cells transformed, but did not incorporate the plasmid with the arabinose gene.
Group 4 did not observe growth in the third plate.