What is competency for a cell?
How might a plasmid contribute to a selective advantage for a bacterial cell?
What is transformation?
Cells are made competent by;
Heat shock
The exogenous DNA used in bacterial transformation can be,
LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;
Which of the following statement is true concerning transformation;
Observe the results above. What gene is present in the engineered plasmid?
Examine the results for group 4. Why would this group see growth without RFP in the third plate?