QUIZ
Biotechnology (Ch. 20)
2 years ago by
Heather Fischer
Save
Copy and Edit
INSTRUCTOR-LED SESSION
Start a live quiz
ASYNCHRONOUS LEARNING
Assign homework
16 questions
Preview
Show answers
  • Question 1
    30 seconds
    Report an issue
    Q.

    The role of restriction enzymes in DNA technology is to

    answer choices

    provide a vector for the transfer of recombinant DNA

    produce cDNA from mRNA

    produce a cut (usually staggered) at specific restriction sites on DNA

    reseal "sticky ends" after base pairing of complementary bases

  • Question 2
    30 seconds
    Report an issue
    Q.

    Yeast has become important in genetic engineering because it

    answer choices

    has plasmids that can be genetically engineered

    facilitates the study of eukaryotic gene regulation and expression

    grows readily and rapidly in the laboratory

    does all of the above

  • Question 3
    30 seconds
    Report an issue
    Q.

    Gel electrophoresis is a means of separating fragments of DNA based on

    answer choices

    charge

    nucleotide sequence

    genetic information

    size

  • Question 4
    60 seconds
    Report an issue
    Q.

    A plasmid has two antibiotic resistance genes, one for ampicillin and one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a human globin gene were cut with the same restriction enzyme. The plasmids and fragments are mixed, treated with ligase, and used to transform bacterial cells. Clones that have taken up the recombinant DNA

    answer choices

    can grow on plates with ampicillin but not with tetracycline

    can grow on plates with tetracycline but not with ampicillin

    can grow on plates with tetracycline and ampicillin

    cannot grow with any antibiotics

  • Question 5
    30 seconds
    Report an issue
    Q.

    Two DNA fragments created by cutting both with the same restriction enzyme can be recombined

    answer choices

    if both DNA fragments come from the same species

    if both DNA fragments come from animals

    if both DNA fragments come from eukaryotes

    regardless of the sources of the DNA

  • Question 6
    45 seconds
    Report an issue
    Q.

    The following segment of DNA has restriction site I and II, which create restriction fragments a, b, and c. Which of the gels produced by electrophoresis would represent the separation and identity of these fragments?

    answer choices
  • Question 7
    30 seconds
    Report an issue
    Q.

    Because Neanderthal individuals died out relatively recently, their bones have not fossilized (turned to stone). It has been possible to isolate bits of DNA from Neanderthal bones, although there isn't much and what is available is in poor condition. What will be your first step in preparing this DNA for analysis?

    answer choices

    reverse transcriptase

    electrophoresis

    DNA fingerprinting

    polymerase chain reaction

  • Question 8
    30 seconds
    Report an issue
    Q.

    You wish to express a eukaryotic gene product in a prokaryotic expression system. What is the advantage of using cDNA as your starting point?

    answer choices

    cDNA does not contain introns

    cDNA is entirely artificial

    cDNA and prokaryotic DNA use the same genetic code

    cDNA is easier to prepare than genomic DNA

  • Question 9
    30 seconds
    Report an issue
    Q.

    Which is the first enzyme used in the production of a recombinant plasmid?

    answer choices

    restriction enzyme

    reverse transcriptase

    DNA ligase

    DNA polymerase

  • Question 10
    30 seconds
    Report an issue
    Q.

    Which is the last enzyme involved in making recombinant plasmids?

    answer choices

    restriction enzymes

    reverse transcriptase

    DNA ligase

    DNA polymerase

  • Question 11
    30 seconds
    Report an issue
    Q.

    Which is the first enzyme used in the production of cDNA?

    answer choices

    restriction enzyme

    reverse transcriptase

    DNA ligase

    DNA polymerase

  • Question 12
    30 seconds
    Report an issue
    Q.

    Which enzyme is used in PCR?

    answer choices

    restriction enzyme

    reverse transcriptase

    DNA ligase

    DNA polymerase

  • Question 13
    30 seconds
    Report an issue
    Q.

    STRs (short tandem repeats) are a valuable tool for

    answer choices

    DNA microarray assays

    acting as probes following gel electrophoresis

    genetic profiling

    RNAi (RNA interference)

  • Question 14
    30 seconds
    Report an issue
    Q.

    Which of the following has the greatest potential for generating different cell types?

    answer choices

    ES cells

    iPS cells

    adult stem cells

    reprogrammed differentiated body cells

  • Question 15
    30 seconds
    Report an issue
    Q.

    A "pharm" animal is a(n)

    answer choices

    transgenic animal that produces large quantities of a pharmaceutical product

    animal used by the pharmaceutical industry to test new medical treatments

    cloned animal that was produced from an adult cell nucleus inserted into an egg

    genetically modified organism (GMO) whose production is permitted in the U.S. but not in the European Union

  • Question 16
    30 seconds
    Report an issue
    Q.

    Which of the following would be useful in signaling the presence of a disease-causing allele even if the gene has not yet been identified?

    answer choices

    RNA interference

    in situ hybridization

    single nucleotide polymorphisms

    RT-PCR

Report an issue
Why show ads?
Report Ad
Enter Code