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- 1. Multiple Choice
The role of restriction enzymes in DNA technology is to
provide a vector for the transfer of recombinant DNA
produce cDNA from mRNA
produce a cut (usually staggered) at specific restriction sites on DNA
reseal "sticky ends" after base pairing of complementary bases
- 2. Multiple Choice
Yeast has become important in genetic engineering because it
has plasmids that can be genetically engineered
facilitates the study of eukaryotic gene regulation and expression
grows readily and rapidly in the laboratory
does all of the above
- 3. Multiple Choice
Gel electrophoresis is a means of separating fragments of DNA based on
charge
nucleotide sequence
genetic information
size
- 4. Multiple Choice
A plasmid has two antibiotic resistance genes, one for ampicillin and one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a human globin gene were cut with the same restriction enzyme. The plasmids and fragments are mixed, treated with ligase, and used to transform bacterial cells. Clones that have taken up the recombinant DNA
can grow on plates with ampicillin but not with tetracycline
can grow on plates with tetracycline but not with ampicillin
can grow on plates with tetracycline and ampicillin
cannot grow with any antibiotics
- 5. Multiple Choice
Two DNA fragments created by cutting both with the same restriction enzyme can be recombined
if both DNA fragments come from the same species
if both DNA fragments come from animals
if both DNA fragments come from eukaryotes
regardless of the sources of the DNA
- 6. Multiple Choice
The following segment of DNA has restriction site I and II, which create restriction fragments a, b, and c. Which of the gels produced by electrophoresis would represent the separation and identity of these fragments?
- 7. Multiple Choice
Because Neanderthal individuals died out relatively recently, their bones have not fossilized (turned to stone). It has been possible to isolate bits of DNA from Neanderthal bones, although there isn't much and what is available is in poor condition. What will be your first step in preparing this DNA for analysis?
reverse transcriptase
electrophoresis
DNA fingerprinting
polymerase chain reaction
- 8. Multiple Choice
You wish to express a eukaryotic gene product in a prokaryotic expression system. What is the advantage of using cDNA as your starting point?
cDNA does not contain introns
cDNA is entirely artificial
cDNA and prokaryotic DNA use the same genetic code
cDNA is easier to prepare than genomic DNA
- 9. Multiple Choice
Which is the first enzyme used in the production of a recombinant plasmid?
restriction enzyme
reverse transcriptase
DNA ligase
DNA polymerase
- 10. Multiple Choice
Which is the last enzyme involved in making recombinant plasmids?
restriction enzymes
reverse transcriptase
DNA ligase
DNA polymerase
- 11. Multiple Choice
Which is the first enzyme used in the production of cDNA?
restriction enzyme
reverse transcriptase
DNA ligase
DNA polymerase
- 12. Multiple Choice
Which enzyme is used in PCR?
restriction enzyme
reverse transcriptase
DNA ligase
DNA polymerase
- 13. Multiple Choice
STRs (short tandem repeats) are a valuable tool for
DNA microarray assays
acting as probes following gel electrophoresis
genetic profiling
RNAi (RNA interference)
- 14. Multiple Choice
Which of the following has the greatest potential for generating different cell types?
ES cells
iPS cells
adult stem cells
reprogrammed differentiated body cells
- 15. Multiple Choice
A "pharm" animal is a(n)
transgenic animal that produces large quantities of a pharmaceutical product
animal used by the pharmaceutical industry to test new medical treatments
cloned animal that was produced from an adult cell nucleus inserted into an egg
genetically modified organism (GMO) whose production is permitted in the U.S. but not in the European Union
- 16. Multiple Choice
Which of the following would be useful in signaling the presence of a disease-causing allele even if the gene has not yet been identified?
RNA interference
in situ hybridization
single nucleotide polymorphisms
RT-PCR
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