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- 1. Multiple Choice
Describe the bacteria phenotype in the +Plasmid AMP/LB plate.The bacteria grew but did not glow in the darkThe bacteria grew AND glowed in the darkThe bacteria did not grow at all - 2. Multiple ChoiceDescribe the bacteria phenotype in the -Plasmid LB plate.The bacteria grew but did not glow in the darkThe bacteria grew AND glowed in the darkThe bacteria did not grow at all
- 3. Multiple ChoiceDescribe the bacteria phenotype in the -Plasmid LB/AMP plate.The bacteria grew but did not glow in the darkThe bacteria grew AND glowed in the darkThe bacteria did not grow at all
- 4. Multiple ChoiceWhat ingredient in the agar causes the bacteria to fluoresce (glow in the dark)?LBArabinoseAmpicillinAgar
- 5. Multiple ChoiceE. Coli rely on which method of gene control (which results in glowing in the dark)?NegativePositive
- 6. Multiple ChoiceWhat is the purpose of calcium in the bacteria transformation lab?It causes the plasmid DNA to be taken into the bacteria cellsIt binds to the negative charges on the phosphate heads in order to neutralize charge.
- 7. Multiple Choice
Definition: Purposefully adding or removing genetic material (usually in a very early embryo)
Genetic Engineering
GMO
Restriction Enzyme
Gel Electrophoresis
- 8. Multiple Choice
Inserting an insulin gene into a bacterial plasmid is an example of:
Gel Electrophoresis
Recombinant DNA
Some voodoo magic
Restriction mapping
- 9. Multiple Choice
The technique used to amplify DNA is called ______
Recombinant DNA
Genetic Engineering
Polymerase Chain Reaction (PCR)
Gene splicing
- 10. Multiple Choice
The picture shows a ___________.
Bacterium
Bacteriophage
HIV virus
Ebola
- 11. Multiple Choice
Which of the following is a reproductive method of bacteria?
Lytic Cycle
Lysogenic Cycle
Conjugation
Transformation
- 12. Multiple Choice
DNA has a slight ___________ charge.
Positive
Negative
- 13. Multiple Choice
Asexual reproduction in bacteria is called:
Conjugation
Binary Fission
Lytic Cycle
Lysogenic Cycle
- 14. Multiple Choice
What kind of antibiotic was used in our bacterial transformation lab?
Penicillin
Arabinose
Ampicillin
- 15. Multiple Choice
Gel Electrophoresis is a technique used to separate DNA fragments based on ________.
Size
Number of A's and G's
Animal species
Criminal suspects
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