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- 1. Multiple ChoiceThe purpose of streaking a plate is to obtainlarge quantities of bacteriabacteria in pure culture
- 2. Multiple ChoiceWhen streaking a plate, primarilyflex and extend the fingersmove from the wristmove your whole arm
- 3. Multiple ChoicePlates should be incubatedagar side upagar side downit doesn't matter
- 4. Multiple ChoiceWhen streaking a plate, you should go back over the last areanever1-2 times3-4 times8-10 times
- 5. Multiple ChoiceWhen streaking a plate, the lid should beplaced on the bench top, top side upplaced on the bench top, inside surface upshould be held in the same hand as the plateshould be held in the opposite hand from the plate
- 6. Multiple ChoiceWhen streaking a plate, the loop should be flamedAt the beginning and endAt the beginning and end and between the first 2 zonesAt the beginning and end and between the second and third zoneAt the beginning and end and between each zone
- 7. Multiple ChoiceGeorge streaks a plate of medium from a broth culture. After incubation, he sees confluent growth in all sectors. Which of the following errors could NOT explain this result?He forgot to pick a colony from the plateHe forgot to flame the loop between sectorsHe picked additional culture from the plate after the first sectorHe crossed over the previous streak too many times at the beginning of a new sector.
- 8. Multiple ChoiceWhich finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer?little finger of the dominant hand (hand holding the loop)little finger of the non-dominant hand (hand holding the sample)forefinger of the dominant hand (hand holding the loop)thumb and forefinger of the non-dominant hand (hand holding the sample)
- 9. Multiple ChoiceThe bacteriology loop should be sterilized in what part of the Bunsen burner flame?in the bright blue conejust above the bright blue coneabove the flameat the base of the flame
- 10. Multiple ChoiceThe loop should be heldstraight up and downat a very slight angleat a fairly steep angleparallel to the work bench
- 11. Multiple ChoiceWhen transferring liquid using a pipette, the tip of the pipette shouldbe in the center of the test tubeshould touch the side of the test tubenever touch the side of the test tuberemain in the air above the tube and never enter the tube.
- 12. Multiple ChoiceWhen transferring liquid aseptically from one tube to another, you shouldremove the cap of the tube with the little finger of the hand holding the pipetteremove the cap of the tube with the little finger of the hand holding the tubeplace the cap on the bench top with the inside upplace the cap on the bench top with the inside down
- 13. Multiple ChoiceWhat is the first thing you should do?put on gloveswash your handssterilize your workspace
- 14. Multiple ChoiceWhat is the last thing you should do?take off your gloveswash your handssterilize your workspace
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