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- 1. Multiple ChoiceWhat is the backbone of DNA made of?Glucose and NitrogenDeoxyribose and NitrogenDeoxyribose and PhosphateDeoxyribose and Sulfate
- 2. Multiple ChoiceWhat kind of bonds hold the DNA molecule together?Phosphate bonds and Hydrogen bondsDipolar bonds and Hydrogen bondsHydrogen bonds an Sulfate bondsCovalent bonds and Hydrogen bonds
- 3. Multiple ChoiceWhich bases pair together in the DNA molecule?A-G and C-TA-T and C-GA-C and G-TA-U and G-C
- 4. Multiple ChoiceWhich best describes an Okazaki fragment?A piece of single strand DNA that gets replicated into double strand DNAA small piece of RNA attached to the DNA strand to start replication.Complementary pieces of DNA that match to the lagging strandA DNA primer that matches the target DNA
- 5. Multiple ChoiceWhich enzyme seals the fragments of DNA into a continuous strand?DNA LigaseDNA PolymeraseHelicaseExonuclease
- 6. Multiple ChoiceWhich of the following excises introns from the mRNA strand?tRNAExonsSpliceosomeRNA Polymerase
- 7. Multiple ChoiceWhich enzyme is responsible for elongating the mRNA strand?DNA polymeraseRNA polymerasePromoter regionRibosome
- 8. Multiple ChoiceWhich enzyme begins DNA replication with a small strand of RNA?HelicasePrimaseDNA LigaseDNA Polymerase
- 9. Multiple ChoiceThe Gene Expert cartridge allows for which of the following?All PCR reagents are contained within one cartridgeMutiplex PCR for multiple targetsPCR amplification of RNA virusesNested PCR
- 10. Multiple ChoiceWhich enzyme adds base pairs to elongate single stranded DNA?ExonucleaseDNA LigasePrimaseDNA polymerase
- 11. Multiple ChoiceWhat is RFLP used for?Detecting PCR productsAmplify nucleic acidsUsed for Eastern blotDNA fingerprinting
- 12. Multiple ChoiceWhich step in PCR is responsible for hybridization?AnnealingInitiationDenaturationExtension
- 13. Multiple ChoiceWhy is Taq used in PCR?It is a specific DNA PrimerIt is a heat stable polymeraseIt is specific to the target probeIt does not need a cofactor
- 14. Multiple ChoiceWhat is RT-PCR used for?To detect PCR products as they are produced.To quantify PCR productsTo detect a viral loadTo amplify RNA viruses
- 15. Multiple ChoiceWhich of the following techniques allows for mutiplexing?Branched DNANested PCRReal time PCRReverse Transcriptase PCR
- 16. Multiple ChoiceWhich of the following results in an amino acid substitution?DeletionInsertionNonsenseMissense
- 17. Multiple ChoiceWhat is the major drawback of the BioFire Film Array?It can only detect one PCR targetIt cannot detect slowgrowing organismsIt can only do one sample per hourIt cannot detect resistance genes
- 18. Multiple ChoiceWhat advantage does the MALDI-TOF provide?It takes minutes instead of days for identification of pathogensIt can detect multiple targetsIt is inexpensiveIt can detect antibiotic resistance
- 19. Multiple ChoiceWhat testing principle is utilized by the MALDI-TOF?PCRRT-PCRMultiplex PCRMass Spectrometry
- 20. Multiple ChoiceIn gel electrophoresis, what separates the DNA fragments?They are separated by an electrical fieldThey are separated by magnetic fieldsThey are separated by binding to the gelThey are separated by the electrical charge of the gel
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