Separation of DNA using electric current is called.....?
Gel Electrophoresis
Chromosome painting
polymerage chain reaction
Restrictive Enzyme Sorting
2. Multiple Choice
45 seconds
1 pt
What is the enzymatic function of restriction enzymes?
To add new nucleotides to the growing strand of DNA.
To join nucleotides during replication.
To join nucleotides during transcription.
To cleave nucleic acids at specific sites.
3. Multiple Choice
1.5 minutes
1 pt
The unpaired nucleotides produced by the action of restriction enzymes are referred to as
sticky end.
base sequence.
single strands.
restriction fragments.
4. Multiple Choice
45 seconds
1 pt
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
5. Multiple Choice
45 seconds
1 pt
What enzyme forms covalent bonds between restriction fragments?
DNA primase
DNA helicase
DNA ligase
DNA polymerase
6. Multiple Choice
45 seconds
1 pt
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium? I) Transform bacteria with recombinant DNA molecule. II) Cut the plasmid DNA using restriction enzymes. III) Extract plasmid DNA from bacterial cells. IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V) Use ligase to seal plasmid DNA to non-plasmid DNA
I, II, IV, III, V
III, II, IV, V, I
II, III, V, IV, I
III, IV, V, I, II
7. Multiple Choice
2 minutes
1 pt
Organisms that have DNA from a different species artificially inserted into their cells are called
bioremediated
transgenic
cultured
a clone
8. Multiple Choice
1 minute
1 pt
GMO stands for
Genetically Made Organism
Growing More Organisms
Genetically Modified Organism
Growing Many Organisms
9. Multiple Choice
3 minutes
1 pt
All of the following are advantages of GMO's except
pest resistant crops
more nutritional foods
unknown long term health effects
creating more human insulin
10. Multiple Choice
3 minutes
1 pt
You have benefited from selective breeding by having more agricultural produce than would have been possible otherwise.
True
False
11. Multiple Choice
3 minutes
1 pt
Based on these results whose blood was found in the blood stain at the crime scene?
Bob
Sue
John
Lisa
12. Multiple Choice
30 seconds
1 pt
Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?
using recombinant DNA to produce human hormones from bacteria
testing parents for genetic disorders before they have children
engineering fruits and vegetables that resist insects and other pests
C. developing ways to identify criminals through DNA fingerprinting
13. Multiple Choice
3 minutes
1 pt
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
It allows the strands of DNA to be cut into various lengths for testing
It restricts the number of base pairs that can be tested in a sample
It makes the testing simpler by moving the strands into the gel faster
It charges the DNA strands
14. Multiple Choice
30 seconds
1 pt
Combining DNA from 2 different organisms is called
Bioengineering
Biotechnology
Recombinant DNA
Genetic Engineering
15. Multiple Choice
30 seconds
1 pt
This picture is an example of what type of DNA cut?
Sticky Ends
Blunt Ends
16. Multiple Choice
30 seconds
1 pt
To amplify (create more) DNA, which process is used?
Gel Electrophorisis
Cloning
Polymerase Chain Reaction (PCR)
Transgenic Process
17. Multiple Choice
30 seconds
1 pt
DNA fingerprinting works because
Most genes are dominant
Most people have DNA that contains repeats
The most important genes are different among most people
No two people, except identical twins, have exactly the same DNA
18. Multiple Choice
30 seconds
1 pt
A new variety of rice called golden rice has been genetically modified to have vitamin A. people in starving countries can eat this rice and receive vitamin A. Without this rice people go blind from vitamin A deficiency. What word(s) below best describes this rice?
Electrophoresis
DNA fingerprint
Genetically modified organism
Clone
19. Multiple Choice
30 seconds
1 pt
In gel electrophoresis, smaller molecules move . . .
really slow
really fast
to the negative side
to the positive side
20. Multiple Choice
1 minute
1 pt
Scientists uncover the remains of a rodent-like creature. They compare its skeleton to various rodents, but need further proof that it is a part of the rodent family. What other evidence would they most likely rely upon?
DNA comparision
a karyotype
diet comparison
the flora and fauna of its habitat
21. Multiple Choice
30 seconds
1 pt
This picture shows how what is made?
Recombinant DNA
PCR
Gel Electrophorisis
Clones
22. Multiple Choice
30 seconds
1 pt
What applications can gel electrophoresis be used for?
Parental Testing
Criminal Investigations
Sequence Endangered Species DNA
All of the above
23. Multiple Choice
30 seconds
1 pt
Which two bear species are most closely related?
Bear 1 and Bear 2
Bear 1 and Bear 3
Bear 2 and Bear 3
There’s no way to tell
24. Multiple Choice
1 minute
1 pt
In which step would the enzyme ligase be used?
A
B
C
D
25. Multiple Choice
1 minute
1 pt
Which of these is the "gene of interest"?
A
B
C
D
26. Multiple Choice
1 minute
1 pt
What would have caused the plasmid (B) to open?
restriction enzyme
ligase
gene therapy
sticky ends
27. Multiple Choice
1 minute
1 pt
In step D, recombinant DNA is being placed back into _____ to be cloned by binary fission.