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- 1. Multiple ChoiceAntibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.TRUEFALSE
- 2. Multiple Choice
When a positive + result occurs in an ELISA test, then one could conclude that...
a + result always means illness
the antibody is present
it is a false + and no illness
an enzyme is present
- 3. Multiple ChoiceWhat is a primary antibody?the second antibody used to detect foreign particleparticle produced by the antigenthe first antibody used to detect foreign particlesecond cell produced by the macrophage
- 4. Multiple ChoiceWhy is a positive and negative control necessary in the setup of ELISA?positive controls are equal to negative controlsELISA is subject to errors; if controls fail the results are untrustworthyELISA is a well run test that typically does not need controlsnegative and positive controls are needed to exclude all results
- 5. Multiple ChoiceWhy is it necessary to wash the samples repeatedly?it is important to keep the lab wells cleanit is important to wash away all bound and unbound antibodies from teh wellsit is important to wash away the unbound antibodieswashing the wells is just solid lab practice
- 6. Multiple ChoiceA serial dilution isthe stepwise saturation of a substance in solution.a dilution that increase by a factor of 1/10 every stepthe stepwise dilution of a substance in solution.a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.
- 7. Multiple ChoiceWhat would be the tube dilution?1/102/101/1001/1000
- 8. Multiple ChoiceWhat would be the final dilution?1/101/1001/10001/10000
- 9. Multiple ChoiceWhat does the symbol μL stand for?nanolitersmillitersmicrolitersmuliters
- 10. Multiple ChoiceFrom what cells do antibodies originate?b cellst cellsprison cellsred blood cells
- 11. Multiple ChoiceWhat is another name for antibodies?helper t cellshemoglobinimmunoglynocistsimmunoglobulins
- 12. Multiple ChoiceDuring an immune response, which of the following is the first cell responder to attack a pathogen's antigen?B cellT cellmacrophageleukocyte
- 13. Multiple ChoiceWhich of the following are commonly tested for using the ELISA method?HIVPregnancyLyme DiseaseAll of these
- 14. Multiple ChoiceDuring the virtual lab (and eventually our ELISA lab), you will prepare a set of dilutions...why?serial dilutions help determine the level of Ab present in a sampleserial dilutions identify the pathogenserial dilutions help determine the level of macrophages presentserial dilutions identify the killer T cells present
- 15. Multiple ChoiceThe basic 4 steps of the ELISA are:
bind sample to well
add primary Ab and wash
add secondary Ab/enzyme and wash
add substrateTRUEFALSE - Answer choicesTagsAnswer choicesTags