Quiz
ELISA
4 years ago by
harrisone
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  • Question 1
    30 seconds
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    Q. Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.
    answer choices
    TRUE
    FALSE
  • Question 2
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    Q.

    When a positive + result occurs in an ELISA test, then one could conclude that...

    answer choices

    a + result always means illness

    the antibody is present

    it is a false + and no illness

    an enzyme is present

  • Question 3
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    Q. What is a primary antibody?
    answer choices
    the second antibody used to detect foreign particle
    particle produced by the antigen
    the first antibody used to detect foreign particle
    second cell produced by the macrophage
  • Question 4
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    Q. Why is a positive and negative control necessary in the setup of ELISA?
    answer choices
    positive controls are equal to negative controls 
    ELISA is subject to errors; if controls fail the results are untrustworthy
    ELISA is a well run test that typically does not need controls
    negative and positive controls are needed to exclude all results
  • Question 5
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    Q. Why is it necessary to wash the samples repeatedly?
    answer choices
    it is important to keep the lab wells clean
    it is important to wash away all bound and unbound antibodies from teh wells
    it is important to wash away the unbound antibodies
    washing the wells is just solid lab practice
  • Question 6
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    Q. A serial dilution is 
    answer choices
    the stepwise saturation of a substance in solution.
    a dilution that increase by a factor of 1/10 every step
    the stepwise dilution of a substance in solution.
    a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.
  • Question 7
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    Q. What would be the tube dilution?
    answer choices
    1/10
    2/10
    1/100
    1/1000
  • Question 8
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    Q. What would be the final dilution?
    answer choices
    1/10
    1/100
    1/1000
    1/10000
  • Question 9
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    Q. What does the symbol μL stand for?
    answer choices
    nanoliters
    milliters
    microliters
    muliters
  • Question 10
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    Q. From what cells do antibodies originate?
    answer choices
    b cells
    t cells 
    prison cells
    red blood cells
  • Question 11
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    Q. What is another name for antibodies?
    answer choices
    helper t cells
    hemoglobin
    immunoglynocists
    immunoglobulins
  • Question 12
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    Q. During an immune response, which of the following is the first cell responder to attack a pathogen's antigen?
    answer choices
    B cell
    T cell
    macrophage
    leukocyte
  • Question 13
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    Q. Which of the following are commonly tested for using the ELISA method?
    answer choices
    HIV
    Pregnancy
    Lyme Disease
    All of these
  • Question 14
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    Q. During the virtual lab (and eventually our ELISA lab), you will prepare a set of dilutions...why?
    answer choices
    serial dilutions help determine the level of Ab present in a sample
    serial dilutions identify the pathogen
    serial dilutions help determine the level of macrophages present
    serial dilutions identify the killer T cells present
  • Question 15
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    Q. The basic 4 steps of the ELISA are:
    bind sample to well
    add primary Ab and wash
    add secondary Ab/enzyme and wash
    add substrate
    answer choices
    TRUE
    FALSE
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