ELISA | Human Anatomy

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ELISA

11th - 12th grade

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Biology

4 years ago by

harrisone

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Question 1
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Q. Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.
answer choices
TRUE
FALSE
TRUE
alternatives
FALSE
answer explanation
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Question 2
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Q.
When a positive + result occurs in an ELISA test, then one could conclude that...
answer choices
a + result always means illness
the antibody is present
it is a false + and no illness
an enzyme is present
a + result always means illness
alternatives
the antibody is present
it is a false + and no illness
an enzyme is present 
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Question 3
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Q. What is a primary antibody?
answer choices
the second antibody used to detect foreign particle
particle produced by the antigen
the first antibody used to detect foreign particle
second cell produced by the macrophage
the second antibody used to detect foreign particle
alternatives
particle produced by the antigen
the first antibody used to detect foreign particle
second cell produced by the macrophage
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Question 4
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Q. Why is a positive and negative control necessary in the setup of ELISA?
answer choices
positive controls are equal to negative controls
ELISA is subject to errors; if controls fail the results are untrustworthy
ELISA is a well run test that typically does not need controls
negative and positive controls are needed to exclude all results
positive controls are equal to negative controls
alternatives
ELISA is subject to errors; if controls fail the results are untrustworthy
ELISA is a well run test that typically does not need controls
negative and positive controls are needed to exclude all results
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Question 5
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Q. Why is it necessary to wash the samples repeatedly?
answer choices
it is important to keep the lab wells clean
it is important to wash away all bound and unbound antibodies from teh wells
it is important to wash away the unbound antibodies
washing the wells is just solid lab practice
it is important to keep the lab wells clean
alternatives
it is important to wash away all bound and unbound antibodies from teh wells
it is important to wash away the unbound antibodies
washing the wells is just solid lab practice
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Question 6
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Q. A serial dilution is
answer choices
the stepwise saturation of a substance in solution.
a dilution that increase by a factor of 1/10 every step
the stepwise dilution of a substance in solution.
a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.
the stepwise saturation of a substance in solution.
alternatives
a dilution that increase by a factor of 1/10 every step
the stepwise dilution of a substance in solution.
a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.
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Question 7
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Q. What would be the tube dilution?
answer choices
1/10
2/10
1/100
1/1000
1/10
alternatives
2/10
1/100
1/1000
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Question 8
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Q. What would be the final dilution?
answer choices
1/10
1/100
1/1000
1/10000
1/10
alternatives
1/100
1/1000
1/10000
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Question 9
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Q. What does the symbol μL stand for?
answer choices
nanoliters
milliters
microliters
muliters
nanoliters
alternatives
milliters
microliters
muliters
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Question 10
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Q. From what cells do antibodies originate?
answer choices
b cells
t cells
prison cells
red blood cells
b cells
alternatives
t cells
prison cells
red blood cells
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Question 11
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Q. What is another name for antibodies?
answer choices
helper t cells
hemoglobin
immunoglynocists
immunoglobulins
helper t cells
alternatives
hemoglobin
immunoglynocists
immunoglobulins
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Question 12
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Q. During an immune response, which of the following is the first cell responder to attack a pathogen's antigen?
answer choices
B cell
T cell
macrophage
leukocyte
B cell
alternatives
T cell
macrophage
leukocyte
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Question 13
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Q. Which of the following are commonly tested for using the ELISA method?
answer choices
HIV
Pregnancy
Lyme Disease
All of these
HIV
alternatives
Pregnancy
Lyme Disease
All of these
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Question 14
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Q. During the virtual lab (and eventually our ELISA lab), you will prepare a set of dilutions...why?
answer choices
serial dilutions help determine the level of Ab present in a sample
serial dilutions identify the pathogen
serial dilutions help determine the level of macrophages present
serial dilutions identify the killer T cells present
serial dilutions help determine the level of Ab present in a sample
alternatives
serial dilutions identify the pathogen
serial dilutions help determine the level of macrophages present
serial dilutions identify the killer T cells present
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Question 15
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Q. The basic 4 steps of the ELISA are:
bind sample to well
add primary Ab and wash
add secondary Ab/enzyme and wash
add substrate
answer choices
TRUE
FALSE
TRUE
alternatives
FALSE
answer explanation
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