With regard to their chromosomes, what is a major difference between prokaryotes and eukaryotes?
Eukaryotes are diploid while prokaryotes are haploid.
Prokaryotes have DNA located in their cytoplasm, while all the DNA in eukaryotic cells is located inside a membrane bound nucleus.
Eukaryotes have linear chromosomes while most prokaryotes contain a single circular chromsome.
Prokaryotes compact their DNA to a high degree while eukaryotes maintain the chromosomes in an uncompacted state.